Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 24, 2026

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

Ultrasensitive Detection of Porcine Epidemic Diarrhea Virus Infections Using Multivalent DNA Nanostructure-Enabled

Saurabh Umrao^1,2,3,4,5, Akhila Naru⁶, Dhanush Gandavadi^1,2,3

¹Department of Bioengineering, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA.

Advanced Healthcare Materials

|June 23, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Fluorescent carbon dot-based biosensor for the rapid and sensitive detection of Escherichia coli DNA.

Scientific reports·2026

Same author

Lectin-Based Antiviral Strategies for Porcine Reproductive and Respiratory Syndrome Virus 2 Infection: Griffithsin Suppresses Viral Replication In Vitro and Reduces Early Viremia In Vivo.

Microorganisms·2026

Same author

Concomitant Infection With Porcine circovirus 2 and Porcine Epidemic Diarrhea Virus Does Not Increase Enteritis Severity in Experimentally Infected Weaned Piglets.

Transboundary and emerging diseases·2026

Same author

Self-adjuvanting α-helical polypeptide simultaneously delivers neoantigen mRNAs and activates dendritic cells to eradicate tumors.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same author

Selective capture and digital counting of intact HIV pseudovirus using designer DNA nets, tethered motion, and photonic resonator interferometric scattering microscopy.

APL bioengineering·2026

Same author

Point of care molecular cancer diagnostics.

Lab on a chip·2026

A new DNA nanostructure lateral flow assay (LFA) enables rapid, ultrasensitive detection of porcine epidemic diarrhea virus (PEDV) on farms. This field-deployable diagnostic tool offers high accuracy for livestock disease surveillance and food security.

Area of Science:

Nanotechnology
Molecular Diagnostics
Veterinary Medicine

Background:

Early detection of livestock viral pathogens is crucial for disease control and food security.
Current diagnostics often require centralized labs and have long turnaround times, hindering on-farm application.
Porcine epidemic diarrhea virus (PEDV) poses a significant threat to swine health and the pork industry.

Purpose of the Study:

To develop a rapid, ultrasensitive, and field-deployable diagnostic assay for PEDV detection on farms.
To leverage DNA nanostructures and aptamers for enhanced diagnostic performance.
To validate the assay's accuracy, sensitivity, and specificity using clinical samples.

Main Methods:

Development of a DNA nanostructure-enabled lateral flow assay (LFA) presenting multivalent aptamers for PEDV detection.

Keywords:

DNA nanostructures aptamer‐based diagnostics lateral flow assay (LFA)multivalent binding porcine epidemic diarrhea virus (PEDV)pre‐clinical PEDV testing

More Related Videos

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Related Experiment Videos

Last Updated: Jun 24, 2026

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Fluorescent Lateral Flow Immunoassay Based on Quantum Dots Nanobeads

Published on: June 28, 2024

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

DNA Virus Detection System Based on RPA-CRISPR/Cas12a-SPM and Deep Learning

Published on: May 10, 2024

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

Utilized net-shaped DNA nanostructures for increased binding avidity.

Tested the assay on pig oral fluid and clinical samples from swine farms.

Main Results:

The DNA-nanostructure LFA achieved a limit of detection (LOD) of ~1.33 ng/mL for recombinant PEDV nucleocapsid protein and ~278 viral copies/test in oral fluid.
Results were obtained within 10 minutes without nucleic acid extraction or amplification.
Clinical sample validation (n=102) showed 97.06% accuracy, 96.92% sensitivity, and 97.30% specificity, with no cross-reactivity to other swine viruses.

Conclusions:

The DNA-nanostructure LFA is a robust, field-deployable platform for rapid and ultrasensitive PEDV detection.
This technology significantly improves upon existing diagnostic limitations for on-farm disease surveillance.
The modular DNA-Net platform is adaptable for detecting other veterinary and zoonotic pathogens, supporting One Health initiatives.