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Published on: June 28, 2019
Microbial arsenate reduction contributes to the enhanced uptake of arsenic by Pteris vittata
Yeping Li1, Xiaojing Wang1, Manxi Xie1
1College of Natural Resources and Environment, Northwest A&F University, Key Laboratory of Plant Nutrition and Agro-environment in Northwest China, Ministry of Agriculture, Xianyang 712100, China.
Abstract:
Pteris vittata, a typical arsenic hyperaccumulator widely used in remediation, has an incompletely understood relationship with microbial arsenic reduction in terms of its arsenic enrichment efficiency. The wild-type and mutant strains without the arsenic-reducing gene were employed to investigate the growth and arsenic enrichment of P. vittata in Yunnan (YN) and Guangxi (GX) soils by a pot experiment. After 60 days of cultivation, compared with the control group, the total biomass of plants treated with wild-type strains and mutant strains increased by 113.81 % and 59.50 % in YN soil, and by 141.86 % and 118.96 % in GX soil, respectively. The arsenic concentration in leaves treated with wild-type strains was 1.29 times (YN) and 1.17 times (GX) higher than that treated with mutant strains. The calculated arsenic enrichment coefficient was 8.07 and 5.18, respectively, under wild-type strain and mutant strain addition. The contribution rate of the arsenic-reducing capacity of bacteria to the promoting effect of wild-type strain on arsenic removal by P. vittata can reach 15.56 %. Arsenic reduction promoted P. vittata's arsenic uptake and contributed to improving its arsenic enrichment efficiency. Above all, microbial arsenic reduction can not only increase the biomass of P. vittata and affect the activity of its antioxidant enzymes but also significantly enhance the arsenic enrichment efficiency of P. vittata. This finding highlights microbial arsenic reduction's important role in enhancing P. vittata's arsenic uptake efficiency and provides a new theoretical foundation for arsenic-contaminated soil microbial remediation.
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