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Updated: Jun 25, 2026

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

Single-Cell Transcriptomics in Triple-Negative Breast Cancer: Implications for Radionuclide Therapy in Precision

Xiaoxiao Xing¹, Huihuan Yu², Songliang Zhang³

¹Department of Surgery, Affiliated Matern&Child Care Hospital of Nantong University, Nantong City, China.

Cancer Biotherapy & Radiopharmaceuticals

|June 24, 2026

Summary

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Targeted Cancer Therapies

Targeted Cancer Therapies

The targeted cancer therapies, also known as “molecular targeted therapies,” take advantage of the molecular and genetic differences between the cancer cells and the normal cells. It needs a thorough understanding of the cancer cells to develop drugs that can target specific molecular aspects that drive the growth, progression, and spread of cancer cells without affecting the growth and survival of other normal cells in the body.
There are several types of targeted therapies against specific...

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This study used single-cell RNA sequencing to map gene expression in triple-negative breast cancer (TNBC), identifying key risk and protective genes. These findings offer a roadmap for developing targeted radionuclide therapies for TNBC precision oncology.

Area of Science:

Oncology
Genomics
Molecular Biology

Background:

Triple-negative breast cancer (TNBC) presents significant clinical challenges due to its aggressive nature and limited treatment options.
Current therapies like chemotherapy and immunotherapy show incomplete efficacy, necessitating novel precision oncology strategies.
Radionuclide therapy offers promise but requires a deeper understanding of TNBC's molecular targets and tumor microenvironment.

Purpose of the Study:

To characterize gene expression profiles and cellular heterogeneity in TNBC using single-cell RNA sequencing (scRNA-seq).
To identify key risk and protective genes within the TNBC ecosystem with potential theranostic relevance.
To establish a foundation for developing targeted radionuclide therapies for TNBC.

Main Methods:

Keywords:

Mendelian randomization precision oncology radionuclide therapy single-cell RNA sequencing theranostics triple-negative breast cancer tumor microenvironment

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Last Updated: Jun 25, 2026

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Studying Triple Negative Breast Cancer Using Orthotopic Breast Cancer Model

Published on: March 20, 2020

Analysis of TNBC scRNA-seq data (GSE155109) using Scanpy for quality control and annotation.
Mendelian randomization (MR) analyses to determine causal links between gene expression (CASP8, RPS23, SLC22A5, CRIPAK, EMB, CTSW) and TNBC risk.
Module scoring, UMAP, diffusion pseudotime analysis, and qRT-PCR validation in cell lines.

Main Results:

Single-cell analysis revealed 18 distinct TNBC clusters, highlighting tumor microenvironment complexity.
Risk genes (EMB, CASP8) were enriched in endothelial cells, while protective gene RPS23 was found in stromal cells.
Gene expression patterns correlated with angiogenic activity and disease progression, validated by qRT-PCR.

Conclusions:

scRNA-seq systematically elucidated TNBC's gene expression and cellular heterogeneity.
Key risk and protective genes with theranostic potential were identified.
These molecular signatures provide a roadmap for radionuclide therapy in TNBC precision oncology.