Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Properties of Organometallic Compounds01:23

Properties of Organometallic Compounds

Organometallic compounds are compounds that contain a carbon–metal bond. Carbon belongs to an organyl group like alkyl, aryl, allyl, or benzyl groups. The metal can be from Group I or Group II of the periodic table, a transition metal, or a semimetal.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Small-Gain-Based Plug-and-Play Distributed Control Framework for DC Microgrids With Decentralized Reconfiguration.

IEEE transactions on cybernetics·2026
Same author

Association between the C-reactive protein-triglyceride-glucose index and incident cardiovascular disease in middle-aged and older adults with arthritis: a nationwide prospective cohort study with hospital-based cross-sectional replication.

Frontiers in immunology·2026
Same author

Efficient dynamic cooperative deployment and task scheduling in multi-UAV-assisted MEC for dense dynamic environments.

Scientific reports·2026
Same author

Charge-Competition AIEgens Induce Mitochondrial Dysfunction for Selective Eradication of <i>Candida albicans</i> while Restoring Vaginal Microbiota.

Journal of microbiology and biotechnology·2026
Same author

Unidirectional spectral singularity lasing in a defective atomic lattice.

Optics express·2026
Same author

Unidirectional reflection lasing via microwave modulation in defective atomic lattice.

Optics express·2026

Related Experiment Video

Updated: Jun 25, 2026

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

Metal-Organic Framework as a Bioorthogonal Catalyst for Gene Editing.

Ziyi Chen1, Xingyu Liu1, Wanyue Sang2

  • 1Key Laboratory of Biomedical Polymers Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.

Journal of the American Chemical Society
|June 24, 2026
PubMed
Summary
This summary is machine-generated.

Researchers engineered metal-organic frameworks (MOFs) with precise catalytic pores for bioorthogonal RNA manipulation in cells. These MOFs enable controlled RNA reactions and gene editing regulation, offering new therapeutic strategies.

More Related Videos

Synthesis, Hemoglobin Encapsulation and Biorthogonal PEGylation in Hierarchically Porous UiO-66 Nanoparticles for Oxygen Delivery Applications
09:24

Synthesis, Hemoglobin Encapsulation and Biorthogonal PEGylation in Hierarchically Porous UiO-66 Nanoparticles for Oxygen Delivery Applications

Published on: May 8, 2026

Related Experiment Videos

Last Updated: Jun 25, 2026

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells
10:07

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

Published on: August 25, 2017

Synthesis, Hemoglobin Encapsulation and Biorthogonal PEGylation in Hierarchically Porous UiO-66 Nanoparticles for Oxygen Delivery Applications
09:24

Synthesis, Hemoglobin Encapsulation and Biorthogonal PEGylation in Hierarchically Porous UiO-66 Nanoparticles for Oxygen Delivery Applications

Published on: May 8, 2026

Area of Science:

  • Materials Science
  • Chemical Biology
  • Biotechnology

Background:

  • Metal-organic frameworks (MOFs) offer tunable structures for various applications.
  • Bioorthogonal chemistry enables reactions within living systems without interference.
  • RNA manipulation is crucial for gene editing and disease treatment.

Purpose of the Study:

  • To design MOFs with molecularly defined catalytic pores for bioorthogonal RNA manipulation.
  • To investigate the influence of MOF structural precision on RNA recognition and conversion.
  • To develop MOF-based catalysts for intracellular RNA reactions and therapeutic applications.

Main Methods:

  • Reticular design and synthesis of MOF libraries with varied Cu cluster geometries and pore dimensions.
  • Small-angle X-ray scattering (SAXS) to study substrate positioning within MOF pores.
  • Synchrotron soft X-ray microscopy for visualizing intracellular MOF localization and integrity.
  • In vitro and in vivo experiments for RNA cleavage, formation, and gene editing regulation.

Main Results:

  • MOFs with dimensionally matched mesopores and Cu(I) clusters efficiently catalyzed RNA bond cleavage and formation in living systems.
  • Pore chemistry was found to govern substrate positioning, enhancing RNA engagement and product release for complete conversion.
  • Intracellular visualization confirmed MOF integrity and bioorthogonal catalytic activity.
  • An amine-functionalized MOF (MOF-248-NH2) demonstrated controlled sgRNA activation for CRISPR/Cas9 gene editing and cardiac disease treatment in a canine model.

Conclusions:

  • Reticular pore engineering is a powerful strategy for creating programmable bioorthogonal MOF catalysts.
  • MOFs can be designed for precise control over RNA manipulation within living cells.
  • This approach holds significant potential for advancing gene editing technologies and developing novel therapeutics.