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The cultivation of environmental microorganisms has long been hindered by the inability to replicate complex native conditions in vitro. The isolation chip (iChip) addresses this limitation by facilitating the growth of previously uncultivable microorganisms through in situ incubation. Designed for high-throughput microbial cultivation, the iChip comprises hundreds of microchambers, each capable of housing a single microbial cell. These microchambers are loaded with a mixture of molten agar and...
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Updated: Jun 25, 2026

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation
07:28

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Published on: November 4, 2021

DNAzyme-Based Core-Shell Hydrogel Microneedle Arrays for In Situ Live Bacterial Detection on Surfaces.

Wei Xue1,2, Tao Sheng2, Yangyang Chang1,2

  • 1Central Hospital of Dalian University of Technology, Dalian 116000, China.

Environmental Science & Technology
|June 24, 2026
PubMed
Summary
This summary is machine-generated.

New DNAzyme-based microneedle arrays offer simple, rapid, in-situ detection of surface bacteria. This innovation enhances food safety and public health surveillance with a sensitive fluorescence readout.

Keywords:
DNAzymebacterial detectioncore−shellhydrogel microneedle arrayin situ

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Area of Science:

  • Biomaterials Science
  • Analytical Chemistry
  • Food Science

Background:

  • Surface bacterial contamination presents significant public health and food safety challenges.
  • Conventional detection methods are often complex, time-consuming, and require laboratory infrastructure.
  • There is a need for rapid, on-site bacterial detection technologies.

Purpose of the Study:

  • To develop a simple and sensitive method for in-situ live bacterial detection on surfaces.
  • To create DNAzyme-based core-shell hydrogel microneedle arrays (DzCS-HMA) for rapid bacterial analysis.
  • To evaluate the performance and applicability of DzCS-HMA in real-world scenarios.

Main Methods:

  • Fabrication of core-shell hydrogel microneedle arrays incorporating DNAzymes and lysozyme.
  • Development of a three-step detection process: surface wetting, microneedle application, and fluorescence readout.
  • Performance evaluation using Escherichia coli, including limit of detection and predictive agreement on various surfaces.

Main Results:

  • DzCS-HMA achieved a detection limit of 71 CFU/mL for Escherichia coli.
  • Demonstrated excellent performance with 100% positive predictive agreement and 87.5% negative predictive agreement on food and environmental surfaces.
  • Successfully applied in spoilage monitoring and multiplex detection of multiple bacterial species.

Conclusions:

  • DzCS-HMA provides a simple, sensitive, and rapid platform for on-site bacterial detection.
  • This technology has significant potential for improving food safety, clinical diagnostics, and public health surveillance.
  • The microneedle array format facilitates easy application and direct fluorescence readout for efficient bacterial monitoring.