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Related Experiment Video

Updated: Jun 25, 2026

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach
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Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach

Published on: April 25, 2025

Spatial multi-omics implicate the interaction between Tpex and B cells in tertiary lymphoid structures after

Jingying Huang1, Yahui Chen2, Peng Deng1

  • 1Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University Guangzhou China.

Cancer Discovery
|June 24, 2026
PubMed
Summary

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Tertiary lymphoid structures (TLSs) harbor precursors of exhausted T cells (Tpex cells) in breast cancer. Within TLSs, B cells invigorate Tpex cells, driving anti-tumor immunity after therapy.

Area of Science:

  • Immunology
  • Cancer Biology
  • Spatial Biology

Background:

  • Tertiary lymphoid structures (TLSs) are crucial in anti-tumor immunity and therapy response.
  • The precise spatial organization and function of TLSs, especially after neoadjuvant therapy, remain incompletely understood.

Purpose of the Study:

  • To comprehensively characterize the spatial pharmacodynamics of TLSs in breast cancer patients post-neoadjuvant therapy.
  • To elucidate the cellular composition and interactions within TLSs at single-cell resolution.

Main Methods:

  • Multifaceted spatial transcriptomic analysis with whole-transcriptome coverage and single-cell resolution.
  • High-throughput spatial proteomics.
  • T-cell receptor sequencing (TCR-seq) integrated with spatial transcriptomics.

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Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
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Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
06:32

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

Published on: August 18, 2023

Related Experiment Videos

Last Updated: Jun 25, 2026

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach
07:16

Three-Dimensional Imaging of Tumor-Bearing Tissue Using the Iterative Bleaching Extends Multiplexity Approach

Published on: April 25, 2025

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
06:32

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

Published on: August 18, 2023

Main Results:

  • Precursors of exhausted T cells (Tpex cells) were identified to preferentially reside within TLSs.
  • Tumor-specific Tpex cells were found within TLSs, with clonally related effector T cells located outside.
  • B cells were identified as nearest neighbors to Tpex cells, facilitating Tpex cell invigoration through ICOSL-ICOS and CD86-CD28 interactions.

Conclusions:

  • Spatial multi-omics reveals a distinct localization of Tpex cells within TLSs.
  • B cell interactions within TLSs are critical for Tpex cell invigoration and anti-tumor immune response.
  • These findings highlight therapy-induced immune evolution within the tumor microenvironment.