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Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Related Experiment Video

Updated: Jun 25, 2026

Compact Quantum Dots for Single-molecule Imaging

Compact Quantum Dots for Single-molecule Imaging

Published on: October 9, 2012

Reversible Quantum Dot-Driven Multiplex Technology for In Situ Subcellular Structure Imaging.

Senke Luo^1,2, Xiaochen Tang^2,3, Yijie Tang⁴

¹Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, P. R. China.

Analytical Chemistry

|June 24, 2026

Summary

This summary is machine-generated.

We developed ssDNA-quantum dot imaging encoding (ssQIE), a novel method for organelle imaging. ssQIE overcomes limitations of traditional methods, enabling enhanced signal amplification and multiplexing for advanced cellular analysis.

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Last Updated: Jun 25, 2026

Compact Quantum Dots for Single-molecule Imaging

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Published on: October 9, 2012

Mapping Molecular Diffusion in the Plasma Membrane by Multiple-Target Tracing (MTT)

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Published on: May 27, 2012

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Visualizing Subcellular Localization of a Protein in the Heart Using Quantum Dots-Mediated Immuno-Labeling Followed by Transmission Electron Microscopy

Published on: September 16, 2022

Area of Science:

* Nanotechnology and Molecular Biology
* Cellular Imaging and Diagnostics

Background:

* Traditional immunofluorescence methods struggle with signal quenching, limited throughput, and complex antibody modification.
* Existing techniques hinder detailed organelle analysis and multiplexed imaging.

Purpose of the Study:

* To introduce ssDNA-quantum dot imaging encoding (ssQIE) as an improved in situ imaging technique.
* To address limitations in antibody modification and fluorescence channel constraints.
* To enable advanced organelle imaging and analysis, particularly in disease states like ferroptosis.

Main Methods:

* Development of ssQIE, combining quantum dots with DNA nanotechnology for antibody labeling.
* Utilizing ssDNA-modified antibodies and quantum dot-DNA complexes for signal capture.
* Comparative analysis of ssQIE against conventional immunofluorescence and CODEX methods.

Main Results:

* ssQIE demonstrated signal amplification and multiplexing capabilities with single-channel excitation.
* The method proved effective for multiple rounds of cyclic imaging under mild conditions.
* Quantum dot optical properties ensured high-quality imaging performance.

Conclusions:

* ssQIE offers a breakthrough solution for antibody modification and fluorescence channel limitations.
* The technique enables detailed in situ imaging and analysis of organelles, including their role in ferroptosis.
* ssQIE highlights the potential of integrating quantum dots and DNA nanotechnology for advanced biological imaging.