Correlation between diaphragmatic electrical activity and clinical respiratory distress in infants: a prospective...

The electrical activity of the diaphragm (EDi), measured via neurally adjusted ventilatory assist (NAVA), reflects neural respiratory drive and may provide an objective marker of respiratory distress in infants. However, its relationship with clinically assessed respiratory distress remains uncertain, particularly across different levels of maturity. To evaluate the correlation between EDi peak values and respiratory distress severity, as measured by the Silverman-Anderson Score (SAS), in infants with NAVA catheters in situ. A prospective observational study was conducted at a tertiary neonatal unit between January 2024 and December 2025. Infants with a NAVA catheter were included. Infants with congenital diaphragmatic anomalies, neuromuscular disorders, significant congenital heart disease, or receiving palliative care were excluded. Respiratory distress was independently assessed by four experienced clinicians using SAS (≤ 3: minimal/no distress; > 3: moderate/severe distress). Simultaneous EDi peak values (μV) were recorded and averaged across breaths. Correlation between EDi and SAS was assessed using Pearson's correlation coefficient. Subgroup analyses were performed by corrected gestational age (cGA < 32 vs ≥ 32 weeks) and weight at assessment (W < 1500 vs ≥ 1500 g). A total of 200 observations from 44 infants were analysed. Median cGA was 24 + 3 weeks (range 22 + 4-39 + 2) and median weight was 580 g (380-3850). Median SAS was 2 (0-7) and median EDi peak was 6.9 μV (0.5-43.6). EDi peak demonstrated a moderate positive correlation with SAS (r = 0.405, p < 0.001). Among infants with SAS > 3, 60% had EDi peak < 15 μV, whereas only 7% of those with SAS ≤ 3 had EDi peak > 15 μV. In infants with SAS > 3, more mature infants (cGA ≥ 32 weeks and W ≥ 1500 g) had significantly higher EDi peaks than less mature infants (p = 0.001).

EDi peak correlates moderately with clinical respiratory distress in infants but demonstrates limited sensitivity in detecting moderate to severe distress, particularly in less mature and lower weight infants. Maturational differences significantly influence EDi responses, suggesting that EDi should be used as an adjunct to, rather than a replacement for, clinical assessment when guiding respiratory support.

• EDi, measured via NAVA, reflects neural respiratory drive in neonates. • Respiratory muscle strength and neural control mature with increasing gestational age.

• EDi peak shows only moderate correlation with SAS; 60% of infants with moderate/severe distress had EDi < 15 μV. • Maturational stage significantly influences EDi response to distress; published 'normal' EDi thresholds should not be used in isolation to guide ventilatory support.