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Related Experiment Video

Updated: Jun 25, 2026

Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts
09:43

Isolation, Culture, and Characterization of Prostate Cancer-Associated Fibroblasts

Published on: August 1, 2025

Prostatic fibroblast reprogramming by Interleukin-30 activates prostate cancer metastasis programs.

Stefania Livia Ciummo1,2, Carlo Sorrentino1,2, Simona Marchetti1,2

  • 1Department of Medicine and Sciences of Aging, "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini, Chieti, 66100, Italy.

Molecular Biomedicine
|June 24, 2026
PubMed
Summary

Interleukin 30 (IL-30) produced by prostate cancer cells reprograms fibroblasts, driving tumor progression and therapeutic resistance. Targeting this IL-30 signaling axis may offer new strategies against aggressive prostate cancer.

Keywords:
Cancer associated fibroblastsCytokinesMetastasisOrgan-on-chipProstate cancerTumor microenvironment

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Last Updated: Jun 25, 2026

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Cancer-Associated Fibroblasts from Mouse Mammary Tumors as Tools for Molecular and Computational Studies
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Published on: July 3, 2025

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Prostate cancer (PC) progression is driven by therapeutic resistance and tumor-stroma interactions.
  • The molecular mechanisms of cancer-fibroblast crosstalk, especially involving Interleukin (IL)-30, are not fully understood.

Purpose of the Study:

  • To elucidate the role of IL-30 in prostate cancer progression and fibroblast reprogramming.
  • To investigate the reciprocal signaling between PC cells and stromal fibroblasts mediated by IL-30.

Main Methods:

  • Utilized cell co-culture models and a two-organ-on-chip platform.
  • Analyzed signaling pathways including AKT, TGF-β/BMP, and epithelial-mesenchymal transition (EMT) regulators.
  • Correlated gene expression with clinical bone metastasis datasets.

Main Results:

  • IL-30 from PC cells reprograms fibroblasts into pro-angiogenic cancer-associated fibroblasts (CAFs) via IL6Rα/gp130 signaling.
  • Fibroblasts enhance PC cell migration and colonization, with IL-30 overexpression potentiating these effects.
  • IL-30 overrides fibroblast-induced suppression of EMT, inducing key PC-associated genes linked to bone metastasis.

Conclusions:

  • Identified a novel IL-30-driven tumor-stroma signaling axis promoting microenvironmental remodeling and metastatic progression in prostate cancer.
  • IL-30 plays a critical role in mediating reciprocal crosstalk that fuels PC aggressiveness and therapeutic resistance.
  • Targeting the IL-30 pathway presents a potential therapeutic strategy to combat advanced prostate cancer.