Related Experiment Video
Updated: Jun 26, 2026
Optimized Minimally Invasive Transscleral Subretinal Injection Technique in Mouse
Published on: July 25, 2025
Precision-Guided Suprachoroidal Injection Using Ultrasound Biomicroscopy and Custom-Length Microneedles
Puranjay Gupta1, Emily Kao1, William F Chadwick1
1Department of Ophthalmology and Visual Sciences, University of Illinois College of Medicine, Chicago, Illinois.
Background And Objective:
Suprachoroidal (SC) injection enables targeted posterior drug delivery but relies on subjective tactile feedback to confirm depth. This can require multiple passes or cause intravitreal delivery, increasing patient discomfort and complication risk. This study evaluates whether ultrasound biomicroscopy (UBM) can guide custom needle selection based on scleral thickness to improve first-pass SC injection success.
Patients And Methods:
Ten ex vivo porcine eyes underwent pre-injection UBM to measure scleral thickness. Custom-length needles (0.5-1.5 mm) were selected accordingly. SC injections were performed under UBM guidance, with imaging confirming entry and injectate spread.
Results:
UBM confirmed successful SC injection in all specimens, with hypoechoic separation between sclera and choroid. Post-injection, scleral thickness increased significantly (1.13 ± 0.13 mm to 2.28 ± 0.19 mm, P < .0001), validating drug delivery.
Conclusion:
UBM-guided needle selection enables accurate SC injection and may reduce failed attempts. This approach could enhance the efficacy of SC delivery and support broader clinical use across posterior segment diseases.
More Related Videos
10:10Ultrahigh Resolution Mouse Optical Coherence Tomography to Aid Intraocular Injection in Retinal Gene Therapy Research
Published on: November 2, 2018
07:13An Alternative and Validated Injection Method for Accessing the Subretinal Space via a Transcleral Posterior Approach
Published on: December 7, 2016