[Validation of the Patulin Analysis in Apple Juice Using the Antibiotic Analytical Method]

Antibiotics and mycotoxins are both substances derived from microorganisms and occasionally share similar chemical properties. Test procedures used for residual antibiotic analysis might potentially be applied to mycotoxin analysis. This study validated a method for analyzing patulin in apple juice by adapting the analytical method for the antibiotic oxytetracycline. Method validation was performed by recovery tests at 0.05 ppm to evaluate the trueness, repeatability (RSD_r), and within-laboratory reproducibility (RSD_wr).The validation results for clarified apple juice and cloudy apple juice exhibited sufficient recovery (91% and 86%) and precision (RSD_r≦4% and RSD_wr≦4%).The interfering peaks were not observed in the chromatograms of blank extracts, indicating sufficient selectivity. The limit of detection was less than 0.01 ppm in both apple juices. This method can help inspect residual patulin in apple juices.