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Related Concept Videos

Scale-Up Processes01:14

Scale-Up Processes

The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
Upstream Processing01:27

Upstream Processing

Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
Bioreactor Controls-III01:22

Bioreactor Controls-III

Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...
ABC Transporters: Importer01:27

ABC Transporters: Importer

ATP-binding cassette or ABC transporters are a class of ATP-driven pumps that hydrolyze ATP to move solutes across the membrane. They can be grouped into importers and exporters. While exporters are present in all domains of life, importers exist only in bacteria and some plants.
In bacteria, based on the number of transmembrane helices and the chemical nature of their substrates, the ABC importers can be divided into three types:
Electron Transport Chain Components01:29

Electron Transport Chain Components

The electron transport chain (ETC) is a crucial metabolic pathway that facilitates energy conversion in prokaryotic and eukaryotic cells. In eukaryotes, the ETC comprises four membrane-associated protein complexes in the inner mitochondrial membrane. In prokaryotes, the ETC in the plasma membrane can vary in composition, with fewer or different complexes depending on the organism and environmental conditions. These complexes transfer electrons from electron donors, such as NADH and FADH2, to...
Production of Antibiotics01:27

Production of Antibiotics

Penicillin, one of the earliest and most widely used antibiotics, is produced industrially by the filamentous fungus Penicillium chrysogenum. Large stirred-tank bioreactors ranging from tens to hundreds of thousands of liters maintain tightly controlled temperature, pH, and dissolved oxygen conditions to support fungal metabolism and maximize antibiotic yield. Penicillin is a secondary metabolite, synthesized primarily during the stationary growth phase, which requires a carefully managed...

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Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles
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Characterization of Membrane Transporters by Heterologous Expression in E. coli and Production of Membrane Vesicles

Published on: December 31, 2019

Transporter engineering in microbial cell factories.

Yajie Cheng1, Xiuchao Xie1, Liang Guo2

  • 1Qinba State Key Laboratory of Biological Resources and Ecological Environment (Incubation), Shaanxi Province Key Laboratory of Bio-resources, Qinba Mountain Area Collaborative Innovation Center of Bioresources Comprehensive Development, School of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, China.

Trends in Biotechnology
|June 24, 2026
PubMed
Summary
This summary is machine-generated.

Transporter engineering enhances microbial cell factories for sustainable biomanufacturing by improving chemical production. This review details advances in transport mechanisms and engineering strategies for industrial applications.

Keywords:
industrial biomanufacturingmembrane transportmetabolic engineeringmicrobial cell factoriessynthetic biologytransporter engineering

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Area of Science:

  • Biotechnology and Synthetic Biology
  • Metabolic Engineering
  • Industrial Microbiology

Background:

  • Microbial cell factories are crucial for sustainable biomanufacturing.
  • Inefficient transmembrane transport limits their industrial application.
  • Transporter engineering offers solutions to overcome these limitations.

Purpose of the Study:

  • To review advances in transporter engineering for microbial cell factories.
  • To analyze challenges and applications of transporter engineering.
  • To support the development of robust biomanufacturing systems.

Main Methods:

  • Literature review of transporter engineering strategies.
  • Analysis of omics, high-throughput screening, and AI applications.
  • Examination of evolutionary trade-offs and engineering bottlenecks.

Main Results:

  • Transporter engineering has progressed from empirical methods to rational design.
  • Key areas include substrate uptake, product efflux, and stress tolerance.
  • Omics, screening, and AI accelerate transporter discovery and optimization.

Conclusions:

  • Transporter engineering is vital for efficient industrial biomanufacturing.
  • Addressing bottlenecks is key to developing robust microbial cell factories.
  • This field supports sustainable production of high-value chemicals.