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Genome-wide association study and KASP development for growth and leaf traits in Populus deltoides
Jiawei Yan1,2, Chunxiao Liu3, Zhongxu Zhang3
1Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China.
BMC Genomics
|June 25, 2026
Summary
Researchers developed 25 new KASP markers for Populus deltoides (poplar) genetic improvement. These markers enable faster, more efficient phenotype-assisted selection in poplar breeding programs.
Area of Science:
- Forestry
- Genetics
- Plant Breeding
Background:
- Populus deltoides is a key timber species for forest genetic breeding.
- Conventional breeding methods are slow and inefficient due to long breeding cycles.
Purpose of the Study:
- To identify molecular markers for accelerating genetic improvement in Populus deltoides.
- To develop efficient tools for phenotype-assisted selection in poplar.
Main Methods:
- Genotyping of 209 P. deltoides accessions using a 60K SNP liquid array.
- Genome-wide association study (GWAS) analyzing 46,031 SNPs and 15 phenotypic traits.
- Development and validation of Kompetitive Allele-Specific PCR (KASP) markers.
Main Results:
- Identified 219 SNPs significantly associated with 15 phenotypic traits.
- Selected 57 target SNPs and 77 candidate genes.
- Developed 48 polymorphic KASP markers, with 25 showing significant phenotypic differences.
Conclusions:
- 25 validated KASP markers are effective tools for phenotype-assisted selection.
- These markers offer efficient molecular resources for accelerating poplar genetic improvement.
- The developed markers support marker-assisted breeding strategies in poplar.
