Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu01:29

Pharmacogenetic Phenotypes: Alterations in Pharmacokinetics, Drug Targets and Biologic Milieu

Genetic variations significantly influence drug response through pharmacokinetics, receptor interactions, and biologic milieu modifications. Pharmacokinetic alterations impact drug metabolism and clearance, affecting efficacy and toxicity. Variants in drug-metabolizing enzymes, such as CYP2C9 and CYP2C19, alter drug activation and elimination. For example, CYP2C9 loss-of-function variants require lower warfarin doses to prevent excessive bleeding, while CYP2C19 variants reduce clopidogrel...
Structure and Function of Platelets01:18

Structure and Function of Platelets

The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000 platelets, with...
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Co-occurring clonal hematopoiesis exhibits strong selection and high leukemia risk.

Nature communications·2026
Same author

Loss of TMEM65 in mice causes mitochondrial disease mediated by mitochondrial Ca<sup>2</sup>.

Nature communications·2026
Same author

Impact of treatment-induced thrombosis on the prognosis of acute lymphoblastic leukaemia: a protocol for a systematic review and meta-analysis.

BMJ open·2026
Same author

Molecular determinants of thrombosis recurrence risk across venous thromboembolism subtypes.

Blood·2025
Same author

Multipopulation GWAS for venous thromboembolism identifies novel loci followed by experimental validation in zebrafish.

Blood advances·2025
Same author

Genome-wide meta-analysis of heavy menstrual bleeding reveals 36 risk loci.

Blood·2025

Related Experiment Video

Updated: Jun 26, 2026

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

DNA Methylation Variation in Blood Cells may Impact Platelet Function.

Jillian Teichman1,2, Ming-Huei Chen1,2, Florian Thibord1,2,3

  • 1The Framingham Heart StudyNational Heart, Lung, and Blood Institute, National Institutes of HealthFraminghamMassachusettsUnited States.

TH Open : Companion Journal to Thrombosis and Haemostasis
|June 25, 2026
PubMed
Summary
This summary is machine-generated.

DNA methylation in blood cells is linked to platelet function. This study reveals significant associations between DNA methylation sites and platelet reactivity, potentially influencing gene regulation in blood cell development.

Keywords:
DNA methylationEWASepigenomemegakaryocytesplatelet reactivity

More Related Videos

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
09:05

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
06:32

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

Published on: May 2, 2025

Related Experiment Videos

Last Updated: Jun 26, 2026

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells
09:46

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics
09:05

Methylation Specific Multiplex Droplet PCR using Polymer Droplet Generator Device for Hematological Diagnostics

Published on: June 29, 2020

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
06:32

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

Published on: May 2, 2025

Area of Science:

  • Epigenetics and Hematology
  • Molecular Biology
  • Genomics

Background:

  • DNA methylation regulates gene expression without altering DNA sequence.
  • The role of DNA methylation in megakaryocyte (MK) development and platelet function is not well understood.
  • Platelets are crucial for hemostasis, and their function is tightly regulated.

Purpose of the Study:

  • To investigate the association between DNA methylation in circulating blood cells and platelet function.
  • To identify specific DNA methylation sites linked to platelet reactivity.
  • To explore the regulatory mechanisms underlying platelet function at the epigenomic level.

Main Methods:

  • Epigenome-wide association study using the Illumina 450K array on DNA from blood cells.
  • Assessment of platelet function using five bioassays measuring response to agonists.
  • Statistical association models to identify links between DNA methylation sites and platelet traits in the Framingham Heart Study cohort (n=1,314).

Main Results:

  • Identified 46 significant associations between 36 DNA methylation sites and platelet function traits (FDR-adjusted p < 0.05).
  • A specific site (cg24267699) upstream of the ABO gene was strongly associated with ristocetin platelet agglutination.
  • Associated genes are involved in transcription factors, granule release, exocytosis, cytoskeleton, and mitochondrial function.
  • DNA methylation sites showed enrichment for regulatory marks in megakaryocytes.

Conclusions:

  • Blood cell DNA methylation is significantly associated with platelet reactivity.
  • Epigenetic modifications may regulate key genes involved in platelet function.
  • These regulatory processes likely occur during early hematopoietic stem cell or megakaryocyte development.