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Sequencing of mRNA from Whole Blood using Nanopore Sequencing
Published on: June 3, 2019
Orthogonal nanopores cross-validation for multiplex single-molecule profiling
Lin-Lin Zhang1, Fan Gao1, Yi-He Wei1
1Molecular Sensing and Imaging Center, School of Chemistry, Nanjing University Nanjing 210023 P. R. China yilunying@nju.edu.cn.
Chemical Science
|June 25, 2026
Summary
A new nanopore multiplex sensor (NMS) enables simultaneous detection of diverse biomolecules for precision health. This breakthrough advances multiomics analysis for complex diseases like lung cancer.
Area of Science:
- Biotechnology
- Nanotechnology
- Molecular Diagnostics
Background:
- Multiplex biomolecule profiling is crucial for precision health.
- Analyzing diverse biomolecules (DNA, RNA, peptides, etc.) simultaneously is challenging due to their varied properties.
Purpose of the Study:
- To develop a novel nanopore multiplex sensor (NMS) for concurrent profiling of multiple biomolecule types.
- To establish a robust and generalizable platform for rapid, parallel biomarker sensing.
Main Methods:
- Utilized a bioelectronic microchip integrated with orthogonal nanopores.
- Employed complementary recognition abilities of nanopores for simultaneous detection.
- Cross-validated the sensor using five major biomolecule types relevant to non-small-cell lung cancer.
Main Results:
- Achieved concurrent profiling of multiple biomolecule types in a single experiment.
- Demonstrated simultaneous detection and relative quantification of five major biomolecule classes.
- Validated the NMS platform's capability for biomarker sensing.
Conclusions:
- The NMS platform overcomes previous limitations in simultaneous multi-analyte detection.
- This technology represents a significant advancement toward single-molecule multiomics.
- The sensor offers a generalizable approach for rapid biomarker discovery and diagnostics.
