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Orthogonal Nanopores Cross-validation For Multiplex Single-molecule Profiling.

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Orthogonal Nanopores Cross-validation For Multiplex Single-molecule Profiling.

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Related Experiment Video

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Orthogonal nanopores cross-validation for multiplex single-molecule profiling.

Lin-Lin Zhang¹, Fan Gao¹, Yi-He Wei¹

¹Molecular Sensing and Imaging Center, School of Chemistry, Nanjing University Nanjing 210023 P. R. China yilunying@nju.edu.cn.

Chemical Science

|June 25, 2026

View abstract on PubMed

Summary

This summary is machine-generated.

A new nanopore multiplex sensor (NMS) enables simultaneous detection of diverse biomolecules for precision health. This breakthrough advances multiomics analysis for complex diseases like lung cancer.

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

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Published on: July 6, 2022

Related Experiment Videos

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Sequencing of mRNA from Whole Blood using Nanopore Sequencing

Published on: June 3, 2019

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Area of Science:

Biotechnology
Nanotechnology
Molecular Diagnostics

Background:

Multiplex biomolecule profiling is crucial for precision health.
Analyzing diverse biomolecules (DNA, RNA, peptides, etc.) simultaneously is challenging due to their varied properties.

Purpose of the Study:

To develop a novel nanopore multiplex sensor (NMS) for concurrent profiling of multiple biomolecule types.
To establish a robust and generalizable platform for rapid, parallel biomarker sensing.

Main Methods:

Utilized a bioelectronic microchip integrated with orthogonal nanopores.
Employed complementary recognition abilities of nanopores for simultaneous detection.
Cross-validated the sensor using five major biomolecule types relevant to non-small-cell lung cancer.

Main Results:

Achieved concurrent profiling of multiple biomolecule types in a single experiment.
Demonstrated simultaneous detection and relative quantification of five major biomolecule classes.
Validated the NMS platform's capability for biomarker sensing.

Conclusions:

The NMS platform overcomes previous limitations in simultaneous multi-analyte detection.
This technology represents a significant advancement toward single-molecule multiomics.
The sensor offers a generalizable approach for rapid biomarker discovery and diagnostics.