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Published on: August 4, 2023
Rotational thromboelastometry analysis of recovered plasma stored for a long period of time
Ifigeneia Vasiliki Kontoteza1, Marianna Politou1, Georgios Dryllis1
1Hematology Laboratory-Blood Bank, Aretaieion Hospital, National and Kapodistrian University of Athens, Athens, Greece.
Background And Objectives:
Although factor VIII (FVIII) is the marker of quality assurance of fresh-frozen plasma (FFP), global tests of coagulation could more accurately reflect the hemostatic profile of FFP. We aimed to evaluate the hemostatic potential of recovered plasma using rotational thromboelastometry and to correlate it with plasma's FVIII activity.
Materials And Methods:
A total of 60 units of recovered plasma stored at-30°C for 11 years (12 units), 7 years (18 units), 3 years (17 units), and 2 years (13 units), were studied using thromboelastometry (ROTEM®, Munich, Germany) without accelerating activators (NATEM assay), a global assay, that evaluates viscoelastic properties during clot formation and lysis. ROTEM results were correlated with FVIII activity. The statistical analysis was performed using the SAS for Windows 9.4 software platform (SAS Institute Inc., NC, U.S.A.).
Results:
Despite the reduction of FVIII, most ROTEM parameters were within normal range (83%, 73%, 76% of plasma units had a normal clotting time, clot formation time and a angle value, respectively, and 100% of plasma units had a normal Lysis Index 30 [LI30] and LI 60 value) regardless of storage time.
Conclusion:
If confirmed in further studies, global methods of coagulation such as thromboelastometry, could assess the hemostatic potential of recovered plasma more precisely.
