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Related Experiment Video

Updated: Jun 26, 2026

iPSC-Derived Epithelial, Mesenchymal, Endothelial, and Immune Cell Co-Culture to Model Airway Barrier Integrity in Lung Health and Disease

iPSC-Derived Epithelial, Mesenchymal, Endothelial, and Immune Cell Co-Culture to Model Airway Barrier Integrity in Lung Health and Disease

Published on: December 6, 2024

Reticular Basement Membrane Remodelling Regulates Bronchial Epithelial Attachment, Barrier Integrity and Inflammatory

Aileen Hsieh^1,2, Jenna Barker-Mulleder^1,2, Chen Xi Yang^1,2

¹Centre for Heart Lung Innovation, St. Paul's Hospital, Vancouver, BC V6Z 1Y6, Canada.

Advances in Respiratory Medicine

|June 25, 2026

Summary

Asthma airway remodelling increases specific collagen types in the reticular basement membrane (RBM). This enhances bronchial epithelial cell (BEC) barrier function and boosts thymic stromal lymphopoietin (TSLP) release, promoting airway inflammation.

Keywords:

TSLP airway remodelling asthma bronchial epithelial cells collagen extracellular matrix reticular basement membrane

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iPSC-Derived Epithelial, Mesenchymal, Endothelial, and Immune Cell Co-Culture to Model Airway Barrier Integrity in Lung Health and Disease

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Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium

Determining Ciliary Function and Membrane Impermeability of the Pseudostratified Lung Airway Epithelium

Published on: February 21, 2025

Area of Science:

Pulmonary Medicine
Cell Biology
Immunology

Background:

Asthma involves airway epithelial dysfunction and reticular basement membrane (RBM) remodelling.
Asthmatic RBM shows increased collagen-I, -III, and fibronectin deposition compared to healthy airways.

Purpose of the Study:

To compare the effects of various extracellular matrix (ECM) proteins on bronchial epithelial cells (BECs) from healthy and asthmatic individuals.
To investigate how RBM remodelling impacts BEC attachment, barrier function, and cytokine release.

Main Methods:

Systematic comparison of collagen-I, -III, -IV, fibronectin, laminin, and BSA on BECs from 6 healthy and 7 asthma donors.
Real-time assessment of epithelial attachment, spreading, and barrier function using electrical cell-substrate impedance sensing over 72 hours.
ELISA analysis of cell culture supernatants for thymic stromal lymphopoietin (TSLP), IL-6, IL-8, and IL-11 release.

Main Results:

BECs from both healthy and asthma donors exhibited faster attachment, spreading, and barrier formation on collagen-I, -III, -IV, and fibronectin compared to laminin and BSA.
BECs cultured on collagen-I and -III produced significantly more TSLP in both control and asthma groups.
No significant effect on IL-6, IL-8, and IL-11 expression was observed across the different ECM proteins.

Conclusions:

RBM remodelling in asthma promotes enhanced epithelial barrier formation.
Increased deposition of collagen-I and -III in the RBM may drive Th2 inflammation via elevated TSLP release from BECs.
These findings highlight a mechanism linking airway remodelling to inflammatory pathways in asthma.