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Related Experiment Video

Updated: Jun 26, 2026

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
08:55

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Published on: May 18, 2018

Intestinal Barrier Dysfunction and Stem Cell Impairment Following Cardiac Surgery in Pigs: A Porcine Model Study.

Haoyang Nian1,2, Yaqi Li1,2, Zhihao Chen1,2

  • 1Department of Animal Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.

Biology
|June 25, 2026
PubMed
Summary

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Cardiac surgery triggers stress responses impacting intestinal health in animals. This study reveals NF-κB pathway activation impairs intestinal stem cells, affecting gut barrier function post-surgery.

Area of Science:

  • Veterinary Medicine
  • Gastroenterology
  • Surgical Research

Background:

  • Cardiac surgery causes systemic stress, potentially disrupting intestinal homeostasis in animal patients.
  • Mechanisms linking surgical stress to intestinal pathophysiology are not well understood.

Purpose of the Study:

  • To investigate postoperative intestinal pathophysiology in a porcine model.
  • To examine the role of the NF-κB pathway and its effect on intestinal stem cell function after cardiac surgery.

Main Methods:

  • Thirty-two pigs were randomized into control and cardiac surgery model groups.
  • Analyzed NF-κB pathway activation, matrix metalloproteinases (MMPs), and intestinal stem cell markers.
  • Assessed organoid development from isolated intestinal stem cells.
Keywords:
NF-κBcardiac surgeryintestinal stem cellsorganoid

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Last Updated: Jun 26, 2026

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia
08:55

Intestinal Stem Cell Isolation and Culture in a Porcine Model of Segmental Small Intestinal Ischemia

Published on: May 18, 2018

Establishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment
08:24

Establishing a Swine Model of Post-myocardial Infarction Heart Failure for Stem Cell Treatment

Published on: May 25, 2020

Main Results:

  • Surgery led to significant NF-κB pathway activation and MMP upregulation in the intestine.
  • Intestinal stem cells showed reduced expression of barrier proteins.
  • Organoids from surgical pigs had impaired proliferation and delayed morphogenesis.

Conclusions:

  • A NF-κB/MMP-mediated mechanism links surgical stress to intestinal barrier dysfunction and stem cell dysregulation.
  • Findings offer insights for improving perioperative care and reducing gastrointestinal complications in animals.
  • Identified pathways may serve as therapeutic targets for veterinary supportive care.