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Updated: Jun 26, 2026
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Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
Published on: April 11, 2018
Transcriptomic Signatures Associated with Doxorubicin Treatment in Liposarcoma Reveal Coordinated Regulatory Patterns
Anas Khaleel1, Sara Khaleel2, Ruqaya Mohammed Ahmed3
1Faculty of Pharmacy and Medical Sciences, University of Petra, Amman 11196, Jordan.
Diseases (Basel, Switzerland)
|June 25, 2026
Summary
Doxorubicin treatment alters gene expression in liposarcoma, impacting pathways like TGF-beta signaling and extracellular matrix remodeling. This study reveals key regulators and transcriptional changes, offering insights into therapeutic response for this soft tissue sarcoma.
Area of Science:
- Oncology
- Genomics
- Bioinformatics
Background:
- Liposarcoma is a soft tissue sarcoma where doxorubicin chemotherapy is standard for advanced disease.
- Limited therapeutic responses necessitate understanding transcriptional adaptation to chemotherapy.
Purpose of the Study:
- To characterize gene expression alterations in liposarcoma under doxorubicin treatment.
- To identify key regulatory pathways and transcriptional changes associated with chemotherapy response.
Main Methods:
- Bioinformatics-driven transcriptomic analysis of publicly available data (GSE12972).
- Differential gene expression analysis and pathway enrichment analysis (Ingenuity Pathway Analysis).
Main Results:
- Identified 365 significantly altered genes (164 upregulated, 201 downregulated) in doxorubicin-treated liposarcoma.
- Highlighted upstream regulators like TGFB1, TNF, and SMARCA4 involved in extracellular matrix remodeling, immune modulation, and EMT-like states.
- Revealed enriched pathways related to liposarcoma tumor microenvironment, fibrosis, and fibroblast growth factor 1 (FGF1) signaling.
Conclusions:
- Provided a systems-level view of transcriptional changes in liposarcoma during doxorubicin treatment.
- Identified potential regulatory networks influencing therapeutic response and disease progression.
- Generated a hypothesis framework for future functional studies in liposarcoma chemotherapy.
