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Galla Chinensis Polyphenol-Loaded Hemostatic Granules for Rapid Hemostasis, Antibacterial Action, and Wound Healing
Ruoxue Guo1,2, Zihan Wu3, Zirui He1,2
1Key Laboratory for Ultrafine Materials of Ministry of Education, School of Materials Science and Engineering, East China University of Science and Technology, Shanghai 200237, China.
Uncontrolled bleeding, coagulation disorders, and infection-related complications still present substantial challenges in emergency medicine and trauma care. Developing multifunctional hemostatic materials represent an effective strategy for addressing clinical hemostasis problems. In this study, Galla chinensis polyphenols, the effective extract of Galla chinensis, were loaded onto calcium alginate-mesoporous silica granules (CMS-GC). The CMS granules were prepared by in situ liquid-phase technology and GC was loaded by impregnation methods. In vitro and in vivo studies showed that CMS-GC not only activate the endogenous coagulation pathway via GC, but also the multi-level interconnected pores of CMS granules can promote the cross-linking of GC with plasma proteins and formation of a three-dimensional network structure, which further enhances the coagulation effect and shortens the blood clotting time to less than 80 s. In rat liver and femoral artery hemorrhage models, CMS-GC significantly shortened hemostasis time and reduced blood loss, demonstrating superior hemostatic performance. Moreover, within the moist environment sustained by alginate, GC mitigates inflammatory responses via its antibacterial and free-radical clearance properties, and synergistically facilitates wound healing. This CMS-GC multifunctional granule provides an efficient new strategy for traumatic bleeding and subsequent repair.
Uncontrolled bleeding, coagulation disorders, and infection-related complications still present substantial challenges in emergency medicine and trauma care. Developing multifunctional hemostatic materials represent an effective strategy for addressing clinical hemostasis problems. In this study, Galla chinensis polyphenols, the effective extract of Galla chinensis, were loaded onto calcium alginate-mesoporous silica granules (CMS-GC). The CMS granules were prepared by in situ liquid-phase technology and GC was loaded by impregnation methods. In vitro and in vivo studies showed that CMS-GC not only activate the endogenous coagulation pathway via GC, but also the multi-level interconnected pores of CMS granules can promote the cross-linking of GC with plasma proteins and formation of a three-dimensional network structure, which further enhances the coagulation effect and shortens the blood clotting time to less than 80 s. In rat liver and femoral artery hemorrhage models, CMS-GC significantly shortened hemostasis time and reduced blood loss, demonstrating superior hemostatic performance. Moreover, within the moist environment sustained by alginate, GC mitigates inflammatory responses via its antibacterial and free-radical clearance properties, and synergistically facilitates wound healing. This CMS-GC multifunctional granule provides an efficient new strategy for traumatic bleeding and subsequent repair.
