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Updated: Jun 26, 2026
10:50
Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
Published on: May 1, 2019
Separation of Monoclonal Antibody Aggregates Using an Analytical Ultrafiltration Technique
Raja Ghosh1, Mrunal Ingawale1, Yves Durocher2
1Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L7, Canada.
Membranes
|June 25, 2026
Summary
Analytical ultrafiltration offers a new method for quantifying monoclonal antibody aggregates, complementing size exclusion chromatography. This technique shows good agreement with the industry standard for aggregate analysis.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Protein Science
Background:
- Size exclusion chromatography (SEC) is the standard for measuring monoclonal antibody (mAb) aggregates.
- Accurate aggregate quantification is crucial for mAb drug safety and efficacy.
Purpose of the Study:
- To introduce and validate an orthogonal analytical ultrafiltration (AUF) technique for detecting and quantifying mAb aggregates.
- To compare the performance of AUF against SEC for mAb aggregate analysis.
Main Methods:
- Developed an AUF method involving sample injection in ultrafiltration mode to collect monomeric mAbs.
- Utilized a backflow mode to recover and detect aggregated mAbs.
- Quantified aggregates based on relative peak areas, similar to liquid chromatography.
Main Results:
- The AUF technique successfully detected and quantified mAb aggregates in tested samples.
- Data generated by AUF showed good agreement with results from SEC.
- The method demonstrated its potential as a viable alternative for aggregate analysis.
Conclusions:
- Analytical ultrafiltration is a promising orthogonal method for mAb aggregate quantification.
- AUF offers a complementary approach to SEC, with comparable results.
- Further investigation into the advantages and limitations of AUF is warranted.
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