Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fungal Phylum Microsporidia01:28

Fungal Phylum Microsporidia

Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...
Fungal Phylum Basidiomycota01:26

Fungal Phylum Basidiomycota

Basidiomycota is a diverse phylum of fungi that includes ecologically significant decomposers such as white rot fungi, symbionts like mycorrhizal fungi, plant pathogens such as rusts and smuts, and edible species like Agaricus bisporus (the common button mushroom). These fungi play crucial roles in nutrient cycling, symbiotic relationships, and even human health. Their defining feature is the basidium, a microscopic club-shaped structure responsible for producing basidiospores.Fruiting Bodies...
Bacterial Phylum Planctomycetes01:26

Bacterial Phylum Planctomycetes

Planctomycetes are a group of morphologically distinct bacteria predominantly classified into two orders: Planctomycetales and Brocadiales. These gram-negative bacteria exhibit unique features, including division by budding and the presence of stalks or appendages. Their cells are often found in rosette arrangements, and they are notable for possessing an S-layer in their cell envelope, which is relatively uncommon among bacteria. Additionally, Planctomycetes frequently exhibit intracellular...
Fungal Phylum Ascomycota01:28

Fungal Phylum Ascomycota

Phylum Ascomycota, a major division within the subkingdom Dikarya, comprises a diverse range of fungal species, including both unicellular yeasts and filamentous molds such as Aspergillus and Penicillium. These fungi thrive in a variety of habitats, from aquatic ecosystems to terrestrial environments, playing crucial ecological and economic roles.Morphology and ReproductionThe defining characteristic of Ascomycetes, commonly referred to as sac fungi, is the ascus—a sac-like structure that...
Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes02:16

Comparing Mitochondrial, Chloroplast, and Prokaryotic Genomes

The present-day mitochondrial and chloroplast genomes have retained some of the characteristics of their ancestral prokaryotes and also have acquired new attributes during their evolution within eukaryotic cells. Like prokaryotic genomes, mitochondrial and chloroplast genomes neither bind with histone-like proteins nor show complex packaging into chromosome-like structures, as observed in eukaryotes. Unlike mitotic cell divisions observed in eukaryotic cells, mitochondria and chloroplasts...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Targeting cancer cell mitochondria with a multivalent source of singlet oxygen.

Smart molecules : open access·2026
Same author

Hypoxia-triggered dichloroacetate delivery with effective reversal of cancer cell reprogramming.

RSC medicinal chemistry·2026
Same author

Endoperoxide delivered singlet oxygen: the future of PDT, without light or oxygen.

RSC medicinal chemistry·2026
Same author

A theranostic endoperoxide agent with targeted singlet oxygen release and concomitant fluorescence signals.

Journal of materials chemistry. B·2026
Same author

Platinum<sup>IV</sup> Complex Enabling Multiple and Potent Tumor Microenvironment Remodeling for Cancer Chemo-Immunotherapy.

Journal of medicinal chemistry·2026
Same author

Benzothiazole-endoperoxide conjugates protect PC12 cells against β-amyloid-induced cell death via singlet oxygen mediated oxidative detoxification of fibrils.

Smart molecules : open access·2025

Related Experiment Video

Updated: Jun 26, 2026

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
06:56

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

Structure-Based Comparative Analysis Reveals the Landscape of Powdery Mildew Secretomes Across Five Genera.

Noman Ali1, Nan Wu1, Engin U Akkaya2

  • 1School of Bioengineering, Dalian University of Technology, Dalian 116024, China.

Pathogens (Basel, Switzerland)
|June 25, 2026
PubMed
Summary

This study reveals the structural diversity of powdery mildew fungi secretomes, identifying conserved protein folds and a distinct RALPH-centered component. Findings offer a framework for understanding these plant pathogens.

Keywords:
AlphaFold2RNase-like proteins associated with haustoria (RALPH)powdery mildewsecretomestructural landscape

More Related Videos

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants
06:09

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

Published on: March 9, 2021

Related Experiment Videos

Last Updated: Jun 26, 2026

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing
06:56

Purification of High Molecular Weight Genomic DNA from Powdery Mildew for Long-Read Sequencing

Published on: March 31, 2017

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure
10:33

Isolation of Culturable Yeasts and Molds from Soils to Investigate Fungal Population Structure

Published on: May 27, 2022

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants
06:09

An Easy and Flexible Inoculation Method for Accurately Assessing Powdery Mildew-Infection Phenotypes of Arabidopsis and Other Plants

Published on: March 9, 2021

Area of Science:

  • Plant Pathology
  • Structural Biology
  • Fungal Genomics

Background:

  • Powdery mildew fungi are significant plant pathogens causing global crop and ecosystem damage.
  • A comprehensive structural understanding of their secreted proteins (secretomes) across different genera is lacking.

Purpose of the Study:

  • To computationally predict and comparatively analyze the secretomes of five powdery mildew genera.
  • To establish a structural landscape of powdery mildew secretomes for future research.

Main Methods:

  • Utilized AlphaFold2 for structure prediction of 7545 secretome candidates from 26 isolates.
  • Performed structural annotation, clustering (Foldseek), network analysis, and comparison with known effector families.
  • Grouped RNase-like proteins associated with haustoria (RALPH) candidates based on structure.

Main Results:

  • Identified a conserved structural core including ribonuclease, immunoglobulin, and glycosidase folds.
  • MoHrip2-like fold was the most prominent shared fold among fungal effectors.
  • Discovered a RALPH-centered network with conserved and lineage-specific communities.
  • Blumeria genus showed structural distinctions with expanded RALPH components.

Conclusions:

  • Established a comparative structural framework for powdery mildew secretomes.
  • Highlighted conserved and genus-specific structural features, particularly within RALPH proteins.
  • Provided insights into the evolution and function of fungal secretomes for targeted studies.