Biomass-Derived Carbon Dots from Guava Leaves Promote Rice Growth and Yield in a Dose-Dependent Manner

Biomass-derived carbon dots (CDs) have attracted increasing attention in agriculture due to their simple synthesis and low environmental impact. In this study, CDs were synthesized from guava (Psidium guajava) leaves using a hydrothermal method (200 °C, 15 h). The particles had an average size of 6.17 nm and a quantum yield of 2.46%, confirming the successful synthesis of fluorescent carbon nanomaterials from the natural precursor. The effects of CDs on rice (Oryza sativa L., variety HT1) were evaluated through both seed treatment and field application. Soaking seeds in a 200 ppm CD solution for 24 h significantly enhanced shoot and root lengths (28.87 mm and 34.00 mm, respectively) among the tested treatments. In field trials, applying CDs at the same concentration also promoted plant growth, as evidenced by improvements in plant height, leaf development, tillering, and flag leaf characteristics. These changes were reflected in yield, with the highest grain yield of 6.13 t ha^-1 at 200 ppm, exceeding that of the control treatment. The observed positive effects may be due to enhanced photosynthetic activity and better control of oxidative processes in plants. Nevertheless, the effect was less pronounced at higher concentrations. This trend suggests a dose-dependent response.