Spectral Characteristics of Dissolved Organic Matter and Their Associations with Heavy Metal Distribution in...

In cold arid regions, the relationships between dissolved organic matter (DOM) characteristics and heavy metal distributions across ice, water, and sediment interfaces remain insufficiently resolved. This study characterized DOM spectral features and examined their associations with measured metal distributions in a typical frozen eutrophic lake using excitation-emission matrices coupled with parallel factor analysis (EEMs-PARAFAC), ultraviolet-visible absorption spectroscopy (UV-Vis), and Fourier-transform infrared spectroscopy (FTIR). Protein-like substances dominated ice DOM, whereas water and sediment-derived DOM contained more humified fluorescent components. Fluorescence indices confirmed a primarily biological origin across all media, with ice showing the highest autochthonous microbial contribution (BIX = 1.23) but the lowest humification (HIX = 0.26), suggesting a greater contribution of recently produced protein-like fluorescent DOM in the ice samples. Water DOM showed the highest average HIX (1.88), followed by sediment-derived DOM (0.61) and ice DOM (0.26). The measured hydrochemical conditions, including weak alkalinity, elevated total dissolved solids (TDS), and locally low dissolved oxygen, provide environmental context for differences in metal distributions. Exploratory Spearman analysis at 17 matched water stations identified the strongest DOM-metal associations for HIX-As (rho = 0.474, p = 0.054) and FI-Zn (rho = 0.471, p = 0.056), indicating that DOM optical properties provide testable indicators of metal-distribution patterns but should be combined with direct binding and speciation measurements for mechanistic confirmation. Because ice was collected in January 2021, whereas water and sediment were collected in October 2020, cross-medium differences are interpreted as between-campaign associations rather than synchronous partitioning. These findings provide a basis for targeted winter monitoring and future binding, speciation, and freeze-concentration experiments in shallow eutrophic lakes.