lncRNA-EBLN3P promotes triple-negative breast cancer metastasis via activating Wnt/β-catenin signaling pathway

To detect the expression of lncRNA endogenous bornavirus-like nucleoprotein (EBLN3P) in triple-negative breast cancer (TNBC) primary cells and tumor tissues. To explore the functional role of EBLN3P in the malignant progression of TNBC.

EBLN3P expression was detected by using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Northern-blot (NB), the influence of EBLN3P to patients´ prognosis was explored by using Kaplan-Meier (K-M) survival analysis. siRNA was used to knockout EBLN3P in TNBC primary cells, 5-ethynyl-2´-deoxyuridine (EdU) and Transwells were used to evaluate changes in proliferation, migration and invasion abilities. RNA-sequence was used to explore changes of signaling pathway.

EBLN3P was aberrantly upregulated in TNBC, especially those patients with distant metastasis. EBLN3P showed a tumor-promoting role, promoted the proliferation, migration and invasion abilities of TNBC by activating Wnt/β-catenin pathway.

EBLN3P might be a potential biomarker TNBC, especially for TNBC with distant metastasis.