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Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Related Experiment Video

Updated: Jun 27, 2026

Identification of Kinesin-1 Cargos Using Fluorescence Microscopy
08:06

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Published on: February 14, 2016

The KIF6-RBP Complex Orchestrates mRNA Transport Required for Sperm Flagellar Assembly.

Chunbo Xie1,2,3, Sibing Yi1, Xinle Lin1

  • 1Institute of Reproduction and Stem Cell Engineering, Xiangya School of Basic Medical Sciences, Central South University, Changsha, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 25, 2026
PubMed
Summary

Kinesin-6 (KIF6) is crucial for male fertility, transporting essential mRNA to sperm flagella. Impaired KIF6 function leads to immotility by disrupting flagellar assembly.

Keywords:
KIF6RNA‐binding proteinasthenozoospermiaflagellar assemblymRNA transport

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Last Updated: Jun 27, 2026

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Transcriptome-Wide Profiling of Protein-RNA Interactions by Cross-Linking and Immunoprecipitation Mediated by FLAG-Biotin Tandem Purification
08:46

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Published on: May 18, 2020

Area of Science:

  • Reproductive Biology
  • Molecular Cell Biology
  • Genetics

Background:

  • Sperm flagellum assembly relies on intraflagellar transport (IFT) of protein cargoes.
  • Mechanisms for delivering flagellar components during late spermiogenesis remain unclear.

Purpose of the Study:

  • Investigate the role of kinesin genes in male infertility.
  • Identify novel factors regulating sperm flagellar biogenesis.

Main Methods:

  • Screened kinesin genes in asthenozoospermic patients.
  • Created and analyzed KIF6-deficient mouse models.
  • Performed multi-omics analyses (mRNA and protein levels).
  • Investigated molecular interactions using co-immunoprecipitation and complex assembly studies.

Main Results:

  • Identified deleterious KIF6 variants in infertile men with immotile sperm.
  • KIF6 deficiency in mice caused infertility and reduced flagellar proteins.
  • KIF6 facilitates mRNA transport of flagellar structural and metabolic proteins via interaction with RBPs.
  • Disrupted KIF6 function impairs mRNA delivery, protein synthesis, and flagellar function.

Conclusions:

  • KIF6 is essential for male fertility by regulating mRNA transport during spermiogenesis.
  • KIF6 utilizes a novel IFT-like pathway involving RBPs to deliver mRNAs for flagellar biogenesis.
  • This study redefines the traditional protein-centric IFT paradigm by highlighting the role of mRNA transport.