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Updated: Jun 27, 2026

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
10:05

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Published on: May 6, 2018

Cyanobacterial fatty acid esters disrupt polymicrobial oral biofilms and exhibit potential interactions with adhesion

Priyam Bharathidasan1, Dhanraj Ganapathy1, Prabhalakshmi Balasubramaniyan1

  • 1Department of Prosthodontics, Saveetha Dental College and Hospital, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Poonamallee, Chennai, Tamil Nadu 600 077, India.

Archives of Oral Biology
|June 25, 2026
PubMed
Abstract

Insights

Bioactive metabolites from Oscillatoria sp. PB602 show significant antimicrobial and anti-biofilm potential against oral pathogens. This discovery offers a promising natural source for developing novel oral healthcare strategies.

Area of Science:

  • Microbiology
  • Natural Product Chemistry
  • Oral Health

Background:

  • Oral biofilm-associated infections pose challenges due to polymicrobial nature and antimicrobial tolerance.
  • These infections are linked to chronic oral diseases, necessitating new therapeutic approaches.

Purpose of the Study:

  • To evaluate the antimicrobial, anti-biofilm, and antioxidant properties of metabolites from Oscillatoria sp. PB602.
  • To assess their efficacy against pathogens involved in complex oral infections.

Main Methods:

  • Methanolic extracts of Oscillatoria sp. PB602 were tested against oral pathogens using agar diffusion and MIC assays.
  • Anti-biofilm activity was assessed via crystal violet staining and CLSM.
  • Metabolite characterization involved GC-MS and FT-IR, with molecular docking for protein interactions.

Main Results:

  • The extract showed concentration-dependent antimicrobial activity, notably against Candida albicans.
  • Significant inhibition of mixed-species biofilms (>88%) and reduction in biofilm biomass (84%) were observed.
  • GC-MS identified fatty acid esters, and docking revealed moderate binding to virulence proteins.

Conclusions:

  • Oscillatoria sp. PB602 possesses potent antimicrobial and anti-biofilm activities.
  • These findings support its potential as a natural source for oral healthcare products.
  • This research aligns with Sustainable Development Goal 3 by advancing preventive oral healthcare.
Keywords:
Anti-biofilm activityCyanobacterial metabolitesOpportunistic Dental PathogensOral biofilmsOral healthSustainable healthcare

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