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Lineage Tracing In Human Cortical Development.

Home
Lineage Tracing In Human Cortical Development.

Related Experiment Video

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

Published on: May 8, 2020

Lineage tracing in human cortical development.

Guohua Yuan¹, Marilyn Steyert¹, Tomasz J Nowakowski²

¹Department of Neurological Surgery, University of California San Francisco, San Francisco, CA, USA.

Current Opinion in Genetics & Development

|June 25, 2026

View abstract on PubMed

Summary

This summary is machine-generated.

Understanding human cortical development relies on lineage tracing. New methods reveal distinct progenitor behaviors and spatial organization in the human brain compared to rodents, offering insights into neurodevelopmental disorders.

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Lineage Tracing and Clonal Analysis in Developing Cerebral Cortex Using Mosaic Analysis with Double Markers (MADM)

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Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Lineage Tracing of Inducible Fluorescently-Labeled Stem Cells in the Adult Mouse Brain

Published on: May 20, 2022

Area of Science:

Developmental Neuroscience
Human Cortical Development
Cellular Diversity

Background:

Progenitor lineage relationships are crucial for understanding human cortical cellular diversity and organization.
Lineage tracing captures cellular history, revealing progenitor fate potential, temporal regulation, and clonal organization.
Existing knowledge gaps in human cortical development necessitate advanced lineage tracing techniques.

Purpose of the Study:

To review advances in lineage tracing technologies for studying human cortical development.
To highlight how these methods reshape our understanding of progenitor behavior and cortical organization.
To explore the role of lineage dynamics in neurodevelopmental disorders.

Main Methods:

Prospective viral barcoding

CRISPR-based evolvable lineage recording

Retrospective inference from endogenous somatic mutations

Main Results:

Human cortical progenitors display expanded fate potential and prolonged temporal control.
Lineage output shows regionally patterned dispersion, differing from rodent models.
Integration of lineage data with molecular profiling and genetic perturbation enables causal dissection.

Conclusions:

Lineage tracing approaches are revolutionizing the study of human cortical development.
Human cortical development exhibits unique features compared to rodent models.
Disrupted lineage dynamics are implicated in neurodevelopmental disorders, suggesting deviations in developmental trajectories.