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Related Concept Videos

Tissue Transplantation01:24

Tissue Transplantation

Tissue transplantation is a significant medical procedure involving the transfer of cells, tissues, or organs from a donor to a recipient, with the primary aim of restoring lost functions. This procedure is crucial in treating a broad spectrum of diseases, including kidney diseases, liver failure, heart disease, and certain types of cancers.
The Biology of Tissue Transplantation
The biology of tissue transplantation hinges on the Major Histocompatibility Complex (MHC) molecules. These molecules...
Kidney Transplant I: Introduction01:28

Kidney Transplant I: Introduction

A kidney transplant is a surgical approach that involves replacing a non-functioning kidney with a healthy one from a donor. This procedure is often a treatment option for end-stage renal disease (ESRD) patients. The method requires careful recipient selection, including evaluating various medical and psychosocial factors. These criteria vary between transplant centers but generally include assessments of the patient's overall health, adherence to medical recommendations, and lifestyle...
Bone Marrow Sampling and Transplants01:22

Bone Marrow Sampling and Transplants

Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
The transplant begins with high doses of chemotherapy and radiation treatment, which aim to destroy the...
Kidney Transplant II: Surgical Procedure01:26

Kidney Transplant II: Surgical Procedure

Preoperative ManagementThe primary goals of preoperative management in kidney transplantation are to optimize the patient’s metabolic state and prepare them for surgery through diet adjustments, necessary dialysis, and tailored medical treatment. This phase also involves comprehensive infection screening and patient education about the surgical procedure and postoperative care to improve outcomes and adherence.Medical ManagementA comprehensive evaluation is required for both the living donor...
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview

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Related Experiment Video

Updated: Jun 27, 2026

Orthotopic Hind Limb Transplantation in the Mouse
07:15

Orthotopic Hind Limb Transplantation in the Mouse

Published on: February 12, 2016

A perspective on ABO incompatible transplantation.

H Kahlyar1, C Hogan2, D Roxby3

  • 1Royal North Shore Hospital, Sydney, Australia.

Human Immunology
|June 25, 2026
PubMed
Summary
This summary is machine-generated.

ABO blood group antibody titration is crucial for ABO-incompatible transplants but lacks standardization, leading to variability. Improving assay reliability is key for better transplant outcomes.

Keywords:
ABO incompatible kidney and stem cell transplantABO incompatible transplantationABO titresIsohaemagglutinin titresTransplantation

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Last Updated: Jun 27, 2026

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Area of Science:

  • Immunology
  • Transplantation Science
  • Clinical Chemistry

Background:

  • ABO blood group compatibility is vital for solid organ and stem cell transplants.
  • ABO-incompatible (ABOi) transplantation faces risks like antibody-mediated rejection (ABMR) due to recipient antibodies binding donor antigens.
  • Isohaemagglutinin measurement via antibody titration is essential for assessing transplant suitability and guiding desensitization.

Purpose of the Study:

  • To review current laboratory challenges in ABO antibody titration.
  • To discuss alternative and evolving analytical approaches for ABO antibody assessment.
  • To explore future directions for improving ABO antibody testing reliability in ABOi transplantation.

Main Methods:

  • Review of existing literature on ABO antibody titration methodologies.
  • Analysis of inter-laboratory variability stemming from diverse assay platforms and techniques.
  • Discussion of alternative methods like flow cytometry, solid-phase assays, and complement-binding tests.

Main Results:

  • Significant inter-laboratory variability exists in ABO antibody titration due to non-standardized methods.
  • Methodological variations, interpretation of titres, and biological factors impact antibody expression and measurement.
  • Current titration methods can influence transplant eligibility and patient outcomes.

Conclusions:

  • Lack of international standardization in ABO antibody titration is a major limitation.
  • Standardization, improved risk stratification, and novel technologies are needed for reliable ABO antibody assessment.
  • International collaboration is essential to enhance ABO antibody testing and improve outcomes in ABOi transplantation.