Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
Autophagic Cell Death01:18

Autophagic Cell Death

Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and pro-apoptotic...
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
Cellular Injury V: Apoptosis and Autophagy01:22

Cellular Injury V: Apoptosis and Autophagy

Cells respond to damage and stress through highly coordinated processes that decide whether they survive or undergo controlled self-destruction. Two major pathways involved in this regulation are apoptosis, a type of programmed cell death, and autophagy, a survival mechanism that helps cells adapt to adverse conditions.ApoptosisApoptosis removes aged or injured cells to maintain tissue balance. During this process, the cell shrinks, chromatin condenses and fragments, and membrane-bound...
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a rapamycin-insensitive companion...
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Lewy bodies are surrounded by granulovacuolar degeneration bodies in dementia with Lewy bodies.

Brain pathology (Zurich, Switzerland)·2026
Same author

From autophagy-lysosomal deficits to neurodegeneration in Niemann-Pick type C1 disease: implications for age-related neurodegenerative disorders.

Frontiers in neuroscience·2026
Same author

DNA methylation-mediated memory of obesity in CD4 T lymphocytes perpetuates immune dysregulation.

EMBO reports·2026
Same author

Nitroalkenes exploit dependence on autophagy-lysosome pathway in PARPi-Resistant triple negative breast cancer.

Cancer letters·2026
Same author

Correction: Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity.

eLife·2026
Same author

Autophagy is required for the development and functionality of lacrimal gland-like organoids.

Stem cell reports·2025

Related Experiment Video

Updated: Jun 27, 2026

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

Rewiring ALS by modulating the autophagy receptor SQSTM1.

Laetitia Aubry1, Viktor I Korolchuk2, Sovan Sarkar3

  • 1CECS/AFM, I-STEM, Corbeil-Essonnes 91100, France; INSERM/UEPS, UMR 861, Paris-Saclay University, I-STEM, Corbeil-Essonnes 91100, France.

Stem Cell Reports
|June 25, 2026
PubMed
Summary

Reverse phenotypic mapping aids drug discovery for genetic disorders. This method identified prazosin, a drug that improves motor neuron and zebrafish models of amyotrophic lateral sclerosis by increasing SQSTM1 expression.

More Related Videos

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

Related Experiment Videos

Last Updated: Jun 27, 2026

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells
09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells
08:35

In Situ Immunofluorescent Staining of Autophagy in Muscle Stem Cells

Published on: June 12, 2017

Area of Science:

  • Neuroscience
  • Genetics
  • Pharmacology

Background:

  • Identifying disease-relevant phenotypes for drug screening in genetic disorders remains challenging.
  • Current methods often struggle to link specific genetic mutations to observable disease characteristics.

Purpose of the Study:

  • To explore reverse phenotypic mapping as a strategy to discover therapeutic gene expression signatures.
  • To identify potential drug candidates for amyotrophic lateral sclerosis (ALS) associated with SQSTM1 haploinsufficiency.

Main Methods:

  • Employed reverse phenotypic mapping to uncover gene expression patterns.
  • Utilized induced pluripotent stem cell (iPSC)-derived motor neurons and a zebrafish model.
  • Tested identified drug candidates for their efficacy in rescuing disease phenotypes.

Main Results:

  • Successfully identified prazosin as a therapeutic agent through reverse phenotypic mapping.
  • Demonstrated that prazosin increases sequestosome-1 (SQSTM1) expression.
  • Showcased the rescue of disease phenotypes in both iPSC-derived motor neurons and a zebrafish model of ALS.

Conclusions:

  • Reverse phenotypic mapping is a viable approach for uncovering therapeutic gene expression signatures.
  • Prazosin shows promise in treating ALS linked to SQSTM1 haploinsufficiency by modulating SQSTM1 levels.
  • This strategy offers a novel avenue for drug discovery in genetic neurological disorders.