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Related Experiment Video

Updated: Jun 27, 2026

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Decoding the Lymphangioleiomyomatosis (LAM) Niche Microenvironment via Integrative Analysis of Single Cell Multiomics

Ken Chen1,2,3, Shuyang Zhao1,3, Minzhe Guo1,4

  • 1The Perinatal Institute and Section of Neonatology, Perinatal and Pulmonary Biology, Cincinnati, OH, USA.

The European Respiratory Journal
|June 25, 2026
PubMed
Summary

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This study maps the human Lymphangioleiomyomatosis (LAM) lung environment, revealing three distinct LAM cell subtypes and associated fibroblasts. This multiomics atlas identifies novel cell states and interactions, offering new therapeutic targets for this rare lung disease.

Area of Science:

  • Pulmonology
  • Oncology
  • Cell Biology

Background:

  • Lymphangioleiomyomatosis (LAM) is a rare lung disease driven by TSC1/TSC2 mutations and mTORC1 hyperactivation.
  • Sirolimus stabilizes lung function but doesn't eliminate LAM cells, highlighting knowledge gaps in the tumor microenvironment and cellular heterogeneity.
  • Understanding LAM's cellular complexity is crucial for developing more effective treatments.

Purpose of the Study:

  • To create the first comprehensive multiomics atlas of the human LAM lung niche.
  • To elucidate LAM cellular heterogeneity and the tumor microenvironment's architecture.
  • To identify novel cell states, interactions, and potential therapeutic targets in LAM.

Main Methods:

  • Integrated single-cell/nucleus RNA-seq, single-nucleus ATAC-seq, and spatial transcriptomics.

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Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
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Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

Published on: August 18, 2023

Related Experiment Videos

Last Updated: Jun 27, 2026

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment
11:00

Visualization, Quantification, and Mapping of Immune Cell Populations in the Tumor Microenvironment

Published on: March 25, 2020

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment
06:32

Multiplex Immunohistochemical Analysis of the Spatial Immune Cell Landscape of the Tumor Microenvironment

Published on: August 18, 2023

  • Deconvoluted the complex architecture of the LAM niche.
  • Validated findings using multimodal imaging technologies.

    • Main Results:

    • Identified three distinct LAM subtypes: LAMCORE1 (smooth muscle-like, mTORC1-hyperactive), LAMCORE2 (fibroblast-like, ECM remodeling), and LAMCORE3 (low transcriptional activity).
    • Uncovered spatially resolved LAM-associated fibroblast (LAF) states (LAF-seed, LAF-niche) orchestrating TGF-β signaling and ECM remodeling.
    • Revealed a structured ecosystem with LAMCORE1 at the core, surrounded by LAFs, LAMCORE2, and reprogrammed immune/epithelial cells.

    Conclusions:

    • This study provides a high-resolution blueprint of the LAM niche microenvironment.
    • Discovered novel LAM cell states and fibroblast subtypes driving disease progression.
    • Identified new therapeutic targets and crosstalk mechanisms within the LAM ecosystem.