Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles

Stem cell-derived exosomes have gained increasing attention due to their therapeutic potential in various diseases. The successful acquisition of exosomes with the desired therapeutic efficacy requires both the appropriate cultivation of the source stem cells and the use of suitable isolation methods. In this context, serum-free culture approaches have emerged as a key strategy to enhance exosome yield. This chapter explains in detail how to properly grow stem cells, how to adapt them to serum-free culture conditions, and how to get exosomes from these cells.