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Related Concept Videos

Oogenesis02:07

Oogenesis

In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...

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Related Experiment Video

Updated: Jun 27, 2026

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
12:36

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

Mice lacking NF-ĸB1 undergo premature ovarian aging.

Carolina Lliberos1, Seng H Liew1, Yasmin Lewis1

  • 1Department of Anatomy and Developmental Biology, Monash Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.

Reproductive Biology and Endocrinology : RB&E
|June 26, 2026
PubMed
Summary

Loss of NF-ĸB1 in mice causes chronic inflammation, accelerating ovarian aging and fertility loss. This suggests a link between inflammation and premature menopause, impacting female reproductive health.

Keywords:
AgingFollicleInflammationNF-ĸBOvaryPremature aging

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Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice
06:49

Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice

Published on: February 12, 2018

Related Experiment Videos

Last Updated: Jun 27, 2026

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse
12:36

Whole Ovary Immunofluorescence, Clearing, and Multiphoton Microscopy for Quantitative 3D Analysis of the Developing Ovarian Reserve in Mouse

Published on: September 3, 2021

Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice
06:49

Orthotopic Ovarian Transplantation Procedures to Investigate the Life- and Health-span Influence of Ovarian Senescence in Female Mice

Published on: February 12, 2018

Area of Science:

  • Reproductive Biology
  • Immunology
  • Aging Research

Background:

  • Female fertility significantly declines with age due to reduced oocyte quantity and quality.
  • This age-related decline leads to ovarian dysfunction, infertility, and menopause.
  • Factors driving natural follicle loss and premature ovarian aging are not fully understood.

Purpose of the Study:

  • To investigate the role of nuclear factor kappa B transcription factor NF-ĸB1 in age-related ovarian reserve depletion.
  • To determine if loss of NF-ĸB1 influences premature ovarian aging and fertility.

Main Methods:

  • Utilized genetically modified mice lacking or having reduced levels of NF-ĸB1 (Nfkb1-/- and Nfkb1±).
  • Assessed ovarian reserve, primordial follicle pool, and corpora lutea counts over time.
  • Measured serum cytokine and intra-ovarian inflammatory markers.

Main Results:

  • Nfkb1-/- mice exhibited accelerated depletion of the ovarian reserve, indicative of premature ovarian aging.
  • Nfkb1± mice also showed early ovarian reserve loss and reduced corpora lutea, suggesting decreased ovulation.
  • Loss of NF-ĸB1 correlated with elevated inflammatory markers, but not overt ovarian fibrosis.

Conclusions:

  • Loss of NF-ĸB1 contributes to chronic low-grade inflammation.
  • This inflammation accelerates age-associated follicle depletion, leading to early fertility loss and premature menopause.
  • NF-ĸB1 deficiency impacts female reproductive lifespan by affecting ovarian reserve maintenance.