Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dexamethasone restores blood-brain barrier integrity in an in vitro heatstroke model.

PloS one·2026
Same author

A cross-strain CRISPRi sgRNA library for <i>Streptococcus agalactiae</i>.

Microbiology resource announcements·2026
Same author

Investigating the Potential Role of Capsaicin in Facilitating the Spread of Coxsackievirus B3 via Extracellular Vesicles.

International journal of molecular sciences·2026
Same author

CRISPR interference in a <i>Streptococcus agalactiae</i> multi-locus sequence type 17 strain.

Journal of bacteriology·2026
Same author

Stem cell-derived brain-like endothelial cells to interrogate <i>Streptococcus pneumoniae</i> interaction with brain endothelium.

Virulence·2025
Same author

CRISPR interference in a <i>Streptococcus agalactiae</i> Multi-locus Sequence Type 17 Strain.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jun 27, 2026

Predicting In Vivo Payloads Delivery using a Blood-brain Tumor-barrier in a Dish
13:34

Predicting In Vivo Payloads Delivery using a Blood-brain Tumor-barrier in a Dish

Published on: April 16, 2019

Modeling Blood-Brain Barrier Efflux Transport Using a Breast Cancer Resistance Protein Overexpression Cell Line.

Alexandra E Meyer1, Natalie G Alexander1,2, Elisa M Tucker1

  • 1Department of Biological Sciences, University of Alabama, Tuscaloosa, AL 35401, USA.

Biomedicines
|June 26, 2026
PubMed
Summary

Researchers developed a new cell model (hCMEC/D3-BCRP) to study Breast Cancer Resistance Protein (BCRP) at the blood-brain barrier (BBB). This model aids in understanding BCRP

Keywords:
ABCG2blood–brain barrierbreast cancer resistance proteindrug deliveryefflux transport

More Related Videos

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
16:26

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport

Published on: June 28, 2014

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier
09:21

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

Published on: November 30, 2021

Related Experiment Videos

Last Updated: Jun 27, 2026

Predicting In Vivo Payloads Delivery using a Blood-brain Tumor-barrier in a Dish
13:34

Predicting In Vivo Payloads Delivery using a Blood-brain Tumor-barrier in a Dish

Published on: April 16, 2019

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
16:26

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport

Published on: June 28, 2014

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier
09:21

A Triple Culture Cell System Modeling the Human Blood-Brain Barrier

Published on: November 30, 2021

Area of Science:

  • Neuroscience
  • Cell Biology
  • Pharmacology

Background:

  • The blood-brain barrier (BBB) protects the central nervous system (CNS) via specialized brain endothelial cells (BECs).
  • Key BEC features include tight junctions, low endocytosis, and efflux transporters like Breast Cancer Resistance Protein (BCRP).
  • BCRP at the BBB is crucial for CNS protection but hinders drug delivery and contributes to multidrug resistance.

Purpose of the Study:

  • To generate a BEC cell line with overexpression of BCRP for further research.
  • To investigate the role and function of BCRP in BECs.
  • To create a tool for studying efflux transport and its impact on drug delivery to the brain.

Main Methods:

  • Utilized the hCMEC/D3 cell line and BCRP substrate rosuvastatin to select for high BCRP expression, creating hCMEC/D3-BCRP.
  • Assessed BCRP protein abundance using flow cytometry and confirmed expression via quantitative PCR (qPCR).
  • Investigated BCRP efflux function by performing substrate accumulation assays with BCRP and P-glycoprotein (P-gp) substrates.

Main Results:

  • The generated hCMEC/D3-BCRP cell line exhibited significantly increased BCRP abundance and expression compared to the parent hCMEC/D3 cells.
  • Functional assays confirmed enhanced BCRP activity in hCMEC/D3-BCRP cells, demonstrated by increased substrate accumulation.
  • The study successfully created a BEC model with overexpressed BCRP.

Conclusions:

  • BCRP plays a vital protective role in the BBB but poses a significant challenge for therapeutic drug delivery to the brain.
  • The novel hCMEC/D3-BCRP cell line, developed using endogenous promoters, serves as a valuable tool for investigating BCRP's function in BECs.
  • This cell line can be instrumental in efflux transport studies and advancing our understanding of CNS drug delivery barriers.