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Related Experiment Video

Updated: Jun 27, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

MicroRNA-30c-1-3p Alleviates Hypoxia-Induced Cardiomyocyte Dysfunction via Tnrc6a Targeting.

Jung-Won Choi1,2, Seongtae Jeong2,3, Seung Eun Jung1,2

  • 1Department of Convergence Science, College of Medicine, Catholic Kwandong University, Gangneung-si 25601, Republic of Korea.

Biomedicines
|June 26, 2026
PubMed
Summary

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...

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This summary is machine-generated.

MicroRNA miR-30c-1-3p protects heart cells from damage caused by low oxygen (hypoxia) after myocardial infarction. This protection involves regulating the Tnrc6a gene, offering potential for new heart attack therapies.

Area of Science:

  • Cardiology
  • Molecular Biology
  • Genetics

Background:

  • Myocardial infarction (MI) is a major cause of death, leading to cardiomyocyte loss via hypoxia.
  • Hypoxia induces mitochondrial dysfunction, oxidative stress, inflammation, and apoptosis, impairing cardiac function.
  • MicroRNAs (miRNAs) regulate cardiomyocyte stress responses, but many hypoxia-responsive miRNAs lack defined functions.

Purpose of the Study:

  • Investigate the role of miR-30c-1-3p, downregulated in early MI, in hypoxia-induced cardiomyocyte injury.
  • Identify and validate downstream targets of miR-30c-1-3p, focusing on Tnrc6a.

Main Methods:

  • Utilized primary neonatal rat cardiomyocytes for gain- and loss-of-function studies.
  • Performed luciferase reporter assays and Tnrc6a knockdown to assess miR-30c-1-3p effects.
Keywords:
Tnrc6acardiomyocytesmiR-30c-1-3pmicroRNAmyocardial infarction

Related Experiment Videos

Last Updated: Jun 27, 2026

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge
09:53

In Vivo Nanovector Delivery of a Heart-specific MicroRNA-sponge

Published on: June 15, 2018

  • Evaluated apoptosis, inflammatory cytokine secretion, and myocardial injury markers.

    • Main Results:

    • Restoring miR-30c-1-3p significantly reduced hypoxia-induced apoptosis and inflammation.
    • Decreased levels of myocardial injury markers were observed with miR-30c-1-3p restoration.
    • Protective effects were linked to the regulation of the miR-30c-1-3p/Tnrc6a axis.

    Conclusions:

    • Identified a novel role for the miR-30c-1-3p/Tnrc6a axis in mitigating hypoxia-induced cardiomyocyte injury.
    • The miR-30c-1-3p/Tnrc6a pathway is a potential therapeutic target for myocardial stress adaptation.