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Related Experiment Video

Updated: Jun 27, 2026

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
11:04

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Published on: October 16, 2013

CD44-Hyaluronan-Dependent Monocyte Rolling.

Marcus Hubbe1,2, Robert H Eibl1,2,3

  • 1Laboratory of Immunology and Vascular Biology, Department of Pathology, Stanford University, Stanford, CA 94305, USA.

International Journal of Molecular Sciences
|June 26, 2026
PubMed
Summary
This summary is machine-generated.

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Monocyte rolling and adhesion depend on specific cell interactions. CD44-hyaluronan interactions mediate monocyte rolling on ECV304 cells, especially when alpha-4 integrin adhesion is not dominant.

Area of Science:

  • Immunology
  • Cell Biology
  • Biophysics

Background:

  • Leukocyte recruitment involves sequential adhesion steps, including rolling and integrin-dependent arrest.
  • VLA-4 and CD44-hyaluronan interactions are implicated in leukocyte rolling and adhesion.

Purpose of the Study:

  • To analyze CD44-hyaluronan-dependent monocyte interactions on ECV304 monolayers.
  • To compare these interactions with alpha-4 integrin-sensitive adhesion on endothelial monolayers.

Main Methods:

  • Adhesion assays and parallel-plate flow chamber experiments were utilized.
  • Monocyte interactions were analyzed on ECV304, HMEC-1, and bEnd.3 monolayers.
  • Blocking antibodies, soluble hyaluronan, and hyaluronidase were used to probe interactions.
Keywords:
CD44HA)VCAM-1VLA-4cell adhesionhyaluronan (hyaluronateleukocyte traffickingmonocyteparallel-plate flow chamberrolling

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Related Experiment Videos

Last Updated: Jun 27, 2026

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System
11:04

Systematic Analysis of In Vitro Cell Rolling Using a Multi-well Plate Microfluidic System

Published on: October 16, 2013

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro
09:16

Simultaneous Study of the Recruitment of Monocyte Subpopulations Under Flow In Vitro

Published on: November 26, 2018

Quantitation of Endothelial Cell Adhesiveness In Vitro
10:24

Quantitation of Endothelial Cell Adhesiveness In Vitro

Published on: June 18, 2015

Main Results:

  • WEHI 78/24 monocytoid cells showed CD44- and hyaluronan-dependent adhesion on ECV304 cells.
  • Adhesion to HMEC-1 and bEnd.3 monolayers was sensitive to alpha-4 integrin blockade.
  • Blocking CD44, adding soluble hyaluronan, or hyaluronidase treatment reduced monocyte adhesion and rolling on ECV304 cells.
  • Primary human monocytes also exhibited CD44-dependent adhesion and rolling on ECV304 cells.

Conclusions:

  • ECV304 cells, derived from T24 cells, serve as a hyaluronidase-sensitive model for studying CD44-hyaluronan interactions.
  • A substrate-dependent hierarchy exists where CD44-hyaluronan-dependent monocyte rolling is observed when alpha-4 integrin adhesion is less dominant.
  • The findings highlight the importance of the cell model used in adhesion assays.