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Related Concept Videos

Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during bone...
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Inhibition of CDK Activity02:34

Inhibition of CDK Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
Bone Remodeling01:40

Bone Remodeling

Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
Anaphase Promoting Complex00:50

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
Hormones and Bone Tissue01:17

Hormones and Bone Tissue

The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...

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Related Experiment Video

Updated: Jun 27, 2026

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

Roxadustat Inhibits Osteoclast Differentiation and Function by Disrupting Cell Cycle Exit.

Afang Li1, Li Zuo1, Luyao Li1

  • 1Department of Nephrology, Peking University People's Hospital, Beijing 100044, China.

International Journal of Molecular Sciences
|June 26, 2026
PubMed
Summary
This summary is machine-generated.

Roxadustat inhibits osteoclast differentiation and bone resorption by promoting precursor proliferation and downregulating p27. This drug may offer dual therapeutic benefits for bone disorders, particularly in chronic kidney disease patients.

Keywords:
bone resorptioncell cyclehypoxia-inducible factorosteoclast differentiationroxadustat

More Related Videos

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
11:11

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Published on: May 19, 2019

Related Experiment Videos

Last Updated: Jun 27, 2026

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
11:52

Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Published on: January 27, 2023

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow
11:11

Isolation, Purification, and Differentiation of Osteoclast Precursors from Rat Bone Marrow

Published on: May 19, 2019

Area of Science:

  • Bone Biology
  • Pharmacology
  • Cellular Differentiation

Background:

  • Bone remodeling requires a balance between osteoclast resorption and osteoblast formation.
  • Roxadustat is known to promote osteoblast differentiation, but its effects on osteoclasts are not well understood.

Purpose of the Study:

  • To investigate the impact of roxadustat on osteoclast differentiation and function in vitro.
  • To elucidate the mechanisms underlying roxadustat's effects on osteoclasts.

Main Methods:

  • Primary murine bone marrow-derived mononuclear cells were used.
  • Osteoclast differentiation was induced with M-CSF and RANKL.
  • Assays included cell viability, TRAP staining, bone resorption, RNA-seq, flow cytometry, immunofluorescence, Western blot, and rescue experiments with abemaciclib (a CDK4/6 inhibitor).

Main Results:

  • Roxadustat suppressed osteoclast differentiation and resorption in a concentration-dependent manner without cytotoxicity.
  • RNA-seq indicated enrichment of cell cycle pathways.
  • Roxadustat paradoxically promoted osteoclast precursor proliferation, evidenced by increased Ki67 and decreased p27 expression.
  • Abemaciclib partially reversed the inhibitory effects of roxadustat on osteoclastogenesis and resorption.

Conclusions:

  • Roxadustat inhibits osteoclast differentiation, potentially by disrupting cell cycle exit and promoting precursor proliferation via p27 downregulation.
  • Roxadustat's dual effects on osteoblasts and osteoclasts suggest therapeutic potential for bone disorders, especially in patients with renal anemia and osteoporosis.
  • Further research is warranted to explore roxadustat's clinical application in bone diseases.