Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...
Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to "Potent, Selective Pyrrolopyrimidine PDE11A4 Inhibitors with Improved Pharmaceutical Properties".

ACS medicinal chemistry letters·2026
Same author

Potent, Selective Pyrrolopyrimidine PDE11A4 Inhibitors with Improved Pharmaceutical Properties.

ACS medicinal chemistry letters·2026
Same author

First Demonstration of <i>In Vivo</i> PDE11A4 Target Engagement for Potential Treatment of Age-Related Memory Disorders.

Journal of medicinal chemistry·2024
Same author

Novel 5-HT<sub>7</sub> receptor antagonists modulate intestinal immune responses and reduce severity of colitis.

American journal of physiology. Gastrointestinal and liver physiology·2024
Same author

First Optimization of Novel, Potent, Selective PDE11A4 Inhibitors for Age-Related Cognitive Decline.

Journal of medicinal chemistry·2023
Same author

Extracellular Acetylated Histone 3.3 Induces Inflammation and Lung Tissue Damage.

Biomolecules·2023

Related Experiment Video

Updated: Jun 27, 2026

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

CRISPR Gene Tagging for Illuminating Endogenous Protein Dynamics.

Nader Afifi1, Dennis Colussi1, Oscar Perez-Leal1

  • 1Moulder Center for Drug Discovery Research, Department of Pharmaceutical Sciences, School of Pharmacy, Temple University, Philadelphia, PA 19140, USA.

International Journal of Molecular Sciences
|June 26, 2026
PubMed
Summary

CRISPR gene tagging allows researchers to study proteins in their natural state by inserting various labels into endogenous genes. This fundamental technology advances quantitative cell biology and translational pharmacology.

Keywords:
CRISPR/Cas9HDRgene tagginghigh-content imagingknock-in

More Related Videos

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
07:23

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

Published on: January 26, 2024

Related Experiment Videos

Last Updated: Jun 27, 2026

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9
14:48

Endogenous Protein Tagging in Human Induced Pluripotent Stem Cells Using CRISPR/Cas9

Published on: August 25, 2018

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster
07:23

One-step CRISPR-based Strategy for Endogenous Gene Tagging in Drosophila melanogaster

Published on: January 26, 2024

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • CRISPR-based gene tagging enables tracking of proteins in their native cellular environment.
  • This technique allows insertion of diverse labels (fluorescent, epitope, etc.) without altering protein expression or dynamics.

Purpose of the Study:

  • To review DNA repair mechanisms utilized in endogenous gene tagging.
  • To discuss advancements in protein tag technologies and experimental strategies.
  • To explore the scalability and applications of CRISPR tagging in various research areas.

Main Methods:

  • Discussion of DNA repair pathways: homology-dependent repair and NHEJ-based integration.
  • Review of emerging CRISPR technologies like prime editing, PASTE, and CRISPR-associated transposases.
  • Analysis of protein tagging technologies including fluorescent proteins, split-reporters, and proximity biotinylation.

Main Results:

  • CRISPR tagging facilitates the study of protein function and dynamics in vivo.
  • New methods are expanding the applicability of tagging to challenging cell types.
  • Scalable approaches are being developed for proteome-wide tagging and drug discovery.

Conclusions:

  • CRISPR gene tagging is a foundational technology for quantitative cell biology and pharmacology.
  • Advancements in CRISPR editing and tagging methods are broadening its scope and efficiency.
  • Endogenous tagging holds significant promise for disease modeling and drug development.