Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

OsMYB94 functions as a master regulator of metabolic pathways associated with rice caryopsis maturation and quality formation.

The New phytologist·2026

Same author

A Review of Research Progress in Rice Anther Culture.

Current issues in molecular biology·2026

Same author

Genetic Dissection of Plant Height Variation Between the Parental Lines of the Elite <i>Japonica</i> Hybrid Rice 'Shenyou 26'.

International journal of molecular sciences·2025

Same author

Phenotypic Identification and Fine-Mapping of the Rice Narrow-Leaf Mutant <i>nal25</i>.

Plants (Basel, Switzerland)·2025

Same author

Adoption value of serum hematological inflammatory markers in early diagnosis of necrotising enterocolitis.

Journal of medical biochemistry·2025

Same author

Unveiling the Genetic Basis Underlying Rice Anther Culturability via Segregation Distortion Analysis in Doubled Haploid Population.

Genes·2023

Search research articles

Related Experiment Video

Updated: Jun 27, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

A Rapid and Reliable qPCR Method for Genetic Purity Testing of BT-Type CMS Rice Seed Lots.

Bilian Hu^1,2, Yuting Dai², Can Cheng²

¹College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China.

Current Issues in Molecular Biology

|June 26, 2026

Summary

A new quantitative PCR assay accurately detects and quantifies maintainer-seed contamination in Boro II (BT) cytoplasmic male sterility (CMS) rice seed lots. This method ensures genetic purity and quality for hybrid rice production.

Keywords:

BT-type cytoplasmic male sterility (CMS)japonica hybrid rice quantitative real-time PCR (qPCR)rice seed genetic purity

More Related Videos

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

Related Experiment Videos

Last Updated: Jun 27, 2026

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

A PCR-based Genotyping Method to Distinguish Between Wild-type and Ornamental Varieties of Imperata cylindrica

Published on: February 20, 2012

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

Area of Science:

Plant breeding
Molecular biology
Agricultural science

Background:

Boro II (BT) cytoplasmic male sterility (CMS) is crucial for japonica hybrid rice production.
Ensuring genetic purity and hybrid seed quality necessitates accurate detection of maintainer-seed contamination in BT-type CMS seed lots.

Purpose of the Study:

To develop a SYBR Green-based quantitative real-time PCR (qPCR) assay for detecting and quantifying maintainer-seed contamination in BT-type CMS seed lots.
To validate the assay's specificity, reproducibility, sensitivity, and accuracy for routine genetic purity testing.

Main Methods:

Development of a SYBR Green-based qPCR assay using maintainer-specific and conserved mitochondrial primers.
Validation of primer specificity against maintainer and CMS lines.
Construction of a standard curve using defined CMS-maintainer seed mixtures (0.1-5% contamination) and conversion of ΔCt values to relative abundance (2^-ΔCt).

Main Results:

The developed qPCR assay demonstrated high specificity, reproducibility, and sensitivity.
A strong linear relationship (R² > 0.99) was observed between 2^-ΔCt values and actual contamination levels.
Performance testing with simulated contamination samples showed accurate quantification with acceptable recovery rates.

Conclusions:

The SYBR Green-based qPCR assay is a rapid, robust, and reliable tool for detecting and quantifying maintainer-seed contamination in BT-type CMS rice.
This method facilitates routine genetic purity testing and quality control in hybrid rice seed production, ensuring higher quality seeds.