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Related Experiment Video

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Human Skeletal Muscle Biopsy Procedures Using the Modified Bergström Technique

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Acute and Chronic High-Intensity Exercise Differentially Regulate the miRNA Biogenesis Pathway in Human Skeletal

Zeyu Wu¹, Eveline S Menezes¹, Natalia de M Lyra E Silva²

¹School of Kinesiology and Health Studies, Queen's University, Kingston, ON K7L 3N6, Canada.

|June 26, 2026

Summary

Exercise impacts microRNA (miRNA) biogenesis pathways in skeletal muscle. Acute high-intensity exercise transiently affected miRNA biogenesis gene expression, while chronic training showed no significant changes in key miRNA biogenesis proteins or miR-133 expression.

Keywords:

exercise miR-133 microRNA biogenesis training

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Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Isolation and Differentiation of Primary Myoblasts from Mouse Skeletal Muscle Explants

Published on: October 15, 2019

Area of Science:

Molecular Biology
Exercise Physiology
Skeletal Muscle Adaptation

Background:

MicroRNAs (miRNAs) are crucial regulators of skeletal muscle adaptation.
The precise influence of exercise on the miRNA biogenesis pathway remains incompletely understood.
This study investigates exercise-induced modulation of miRNA biogenesis components and expression.

Purpose of the Study:

To examine the impact of acute and chronic high-intensity exercise on miRNA biogenesis.
To determine if exercise-induced changes in biogenesis components correlate with miRNA expression levels.
To analyze miRNA biogenesis across different stages, from primary to mature transcripts.

Main Methods:

Analysis of muscle biopsies from participants undergoing high-intensity interval cycling exercise (HIIE) and high-intensity interval training (HIIT).
Quantification of mRNA expression for miRNA biogenesis genes (Drosha, Exportin-5, Dicer, Ago2) post-HIIE using RT-qPCR.
Measurement of protein abundance for biogenesis factors post-HIIT via Western blotting and assessment of primary/mature miR-133 expression.

Main Results:

Acute HIIE significantly decreased Drosha mRNA expression and trended towards reducing Dicer mRNA.
Six weeks of HIIT did not yield significant changes in the protein levels of Drosha, Exportin-5, Dicer, or Ago2.
HIIT did not alter the expression of miR-133 at either the primary or mature transcript levels.

Conclusions:

Skeletal muscle miRNA regulation in response to exercise is complex.
Further research is needed to elucidate the temporal and mechanistic control of miRNA biogenesis during exercise.
Exercise effects on miRNA biogenesis may be transient or context-dependent.