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Related Concept Videos

Lymphoid Cells and Tissues01:18

Lymphoid Cells and Tissues

Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Primary Lymphoid Organs01:16

Primary Lymphoid Organs

Primary lymphoid organs are pivotal in the formation, development, and maturation of lymphocytes, the white blood cells that serve as the backbone of our immune system. This crucial function underscores their fundamental role in maintaining our overall health and immunity. The two primary lymphoid organs of prime importance are the red bone marrow and the thymus.
The red bone marrow is a soft, spongy tissue nestled in the interior of long bones such as the humerus and femur. It is the site...

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Related Experiment Video

Updated: Jun 27, 2026

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling
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Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling

Published on: October 14, 2021

A Staged Whole-Blood Transcriptomic Framework Identifies a Compact Myeloid-Lymphoid Activity Score in Systemic Lupus

Chuanwei Zhang1, Lijun Pang1, Ziheng Zhu1

  • 1Department of Rheumatology, The First Affiliated Hospital of Anhui University of Chinese Medicine, Hefei 230038, China.

Genes
|June 26, 2026
PubMed
Summary

This study developed a new transcriptomic framework for systemic lupus erythematosus (SLE) to create a compact, biologically interpretable activity score. The validated score effectively distinguishes SLE patients and disease activity levels using peripheral blood.

Keywords:
BloodGen3compact transcriptomic scoreimmune-state stratificationmolecular endotypessystemic lupus erythematosuswhole-blood transcriptomics

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Published on: November 1, 2015

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Last Updated: Jun 27, 2026

Isolating Human Peripheral Blood Mononuclear Cells and CD4+ T cells from Sézary Syndrome Patients for Transcriptomic Profiling
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The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice
12:04

The bm12 Inducible Model of Systemic Lupus Erythematosus (SLE) in C57BL/6 Mice

Published on: November 1, 2015

Area of Science:

  • Immunology
  • Genomics
  • Transcriptomics

Background:

  • Peripheral-blood transcriptomic profiling offers insights into systemic lupus erythematosus (SLE) molecular heterogeneity.
  • Discovery-stage signatures often lack transportability across different cohorts and validation stages.
  • A need exists for a robust and reproducible transcriptomic framework for SLE research.

Purpose of the Study:

  • To establish a staged whole-blood transcriptomic framework for SLE.
  • To derive a compact, biologically interpretable, and validated activity score for SLE.
  • To improve the transportability and reproducibility of transcriptomic signatures in SLE.

Main Methods:

  • Organized public whole-blood transcriptome cohorts into discovery, public validation, and single-cell reference layers.
  • Employed peripheral blood mononuclear cell (PBMC) reverse transcription quantitative PCR (RT-qPCR)/flow-cytometric analysis and an expanded whole-blood RT-qPCR validation set.
  • Compressed candidate genes into a 6-gene panel (pre6-balanced) selected through staged public validation.

Main Results:

  • Identified two whole-blood endotypes characterized by lymphoid versus myeloid/neutrophil-inflammatory polarization.
  • The pre6-balanced score demonstrated significant discrimination between SLE patients and healthy controls (AUC 0.838-0.888) and between high and low/moderate disease activity (AUC 0.719-0.918).
  • The score showed strong associations with SLE disease activity index (SLEDAI) and complement levels.

Conclusions:

  • A staged framework successfully yielded a compact myeloid-lymphoid activity score validated across multiple layers.
  • The derived score serves as a research-grade relative activity measure for SLE.
  • Prospective evaluation of this score in SLE is warranted.