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Deep Eutectic Solvent-Based Extraction Optimization, Structural Characterization, and Alleviating Effects of Tremella
Zhenhua Fan1, Qiuyun Li2, Weiliang Wu1
1Food Safety and Health Research Center, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou 510515, China.
Foods (Basel, Switzerland)
|June 26, 2026
Summary
A new deep eutectic solvent (DES) method efficiently extracts Tremella fuciformis polysaccharides (TFPS), improving yield and preserving structure. The purified TFPS-1 fraction shows anti-inflammatory effects, alleviating experimental colitis in mice.
Area of Science:
- Food Science
- Biochemistry
- Pharmacology
Background:
- Tremella fuciformis polysaccharides (TFPS) possess anti-inflammatory and gut-modulating properties.
- Conventional extraction methods for TFPS are inefficient and can compromise polysaccharide integrity.
- Deep eutectic solvents (DES) offer a potentially greener and more efficient extraction alternative.
Purpose of the Study:
- To optimize a DES-based extraction method for TFPS.
- To characterize the major TFPS fraction obtained.
- To evaluate the therapeutic potential of TFPS-1 in a dextran sulfate sodium (DSS)-induced colitis model.
Main Methods:
- Single-factor testing and response surface methodology were used to optimize DES extraction parameters (choline chloride-lactic acid).
- The purified TFPS-1 fraction was characterized using chromatographic, spectroscopic, methylation, and NMR analyses.
- Biological efficacy was assessed in DSS-treated mice, monitoring weight loss, colon length, histology, mucus secretion, gene expression, cytokine levels, and gut microbiota.
Main Results:
- Optimized DES extraction significantly increased TFPS yield by 77.6% compared to hot-water extraction.
- TFPS-1 was identified as a low-molecular-weight glucan with specific glucose linkages.
- TFPS-1 treatment reduced colitis symptoms, improved gut barrier function, decreased inflammation, increased IL-10, and modulated gut microbiota in mice.
Conclusions:
- DES-based extraction is an efficient and advantageous method for obtaining TFPS.
- TFPS-1 demonstrates significant therapeutic potential for intestinal protection in experimental colitis.
- TFPS-1 warrants further investigation as a functional food ingredient for managing inflammatory bowel conditions.
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