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Solid Lipid Nanoparticles (SLNs) for Intracellular Targeting Applications

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Silibinin-Loaded Proniosomal Gel for Cutaneous Application: Pharmaco-Technical Characterization and In Vitro-In Ovo

Andreea Smeu^1,2,3, Ioana Olariu⁴, Iasmina Marcovici^2,3

¹Doctoral School, "Victor Babes" University of Medicine and Pharmacy, Eftimie Murgu Square No. 2, 300041 Timisoara, Romania.

Gels (Basel, Switzerland)

|June 26, 2026

Summary

This study developed a novel proniosomal gel for topical silibinin (SIL) delivery. The SIL-loaded proniosomal gel (SIL-PG) shows promising skin-protective properties and a good safety profile for dermal applications.

Keywords:

irritant potential keratinocytes pharmaco-technical evaluation proniosomal gel reconstructed human tissue safety profile silibinin spheroids

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Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Preparation and Characterization of Nanoliposomes for the Entrapment of Bioactive Hydrophilic Globular Proteins

Published on: August 31, 2019

Area of Science:

Dermatology and Pharmaceutical Sciences
Nanotechnology in Drug Delivery
Natural Product Chemistry

Background:

The skin's role as a primary defense barrier makes it susceptible to damage.
Silibinin (SIL) possesses skin-protective benefits, but effective topical delivery is challenging.
Proniosomal platforms for natural products are gaining interest, yet SIL delivery via this method is underexplored.

Purpose of the Study:

To develop and characterize a silibinin-loaded proniosomal gel (SIL-PG) for enhanced skin application.
To evaluate the pharmaco-technical properties and preclinical safety of the SIL-PG formulation.
To assess the potential of SIL-PG for cutaneous delivery of silibinin.

Main Methods:

Proniosomal gel (SIL-PG) preparation using coacervation phase separation.
Physicochemical and technological property analysis of SIL-PG.
In vitro and in ovo evaluation of cytotoxicity and irritant potential (HaCaT cells, 3D EpiDerm™, chorioallantoic membrane).

Main Results:

SIL-PG exhibited a desirable creamy texture, appropriate rheology, and spreadability.
The formulation demonstrated gradual in vitro drug release and sustained skin permeation.
SIL-PG showed no cytotoxicity in keratinocytes or spheroids and no irritant effects in reconstructed human tissues or on the chorioallantoic membrane.

Conclusions:

SIL-PG presents favorable pharmaco-technical characteristics for dermal application.
The formulation possesses a good safety profile, indicated by in vitro and in ovo biocompatibility.
SIL-PG is a promising pharmaceutical candidate for topical silibinin delivery and skin protection.