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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

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Correction: Kang et al. Fluid Flow to Electricity: Capturing Flow-Induced Vibrations with Micro-Electromechanical-System-Based Piezoelectric Energy Harvester. <i>Micromachines</i> 2024, <i>15</i>, 581.

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Related Experiment Video

Updated: Jun 27, 2026

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
10:43

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas

Published on: July 21, 2023

Single-Molecule Detection Concepts Enabled by DNA Origami.

Seppe Driesen1, Karen Leirs1, Jeroen Lammertyn1

  • 1Department of Biosystems, Biosensors Group, KU Leuven, Willem de Croylaan 42, 3001 Leuven, Belgium.

Micromachines
|June 26, 2026
PubMed
Summary
This summary is machine-generated.

DNA origami enables precise nanoarchitecture engineering for single-molecule detection. This review explores DNA nanostructures and readout strategies, highlighting advancements and future directions in this field.

Keywords:
DNA nanotechnologyDNA origamidiagnosticsfluorescencesingle-molecule

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Last Updated: Jun 27, 2026

Single-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas
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DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications
08:59

DNA Origami-Mediated Substrate Nanopatterning of Inorganic Structures for Sensing Applications

Published on: September 27, 2019

Area of Science:

  • Nanotechnology
  • Biophysics
  • Molecular Engineering

Background:

  • DNA origami, since 2006, allows precise fabrication of 2D and 3D DNA nanostructures.
  • These nanostructures serve as programmable nanobreadboards for arranging biomolecules and nanoparticles.
  • This programmability is key for developing DNA nanomachines for single-molecule detection.

Purpose of the Study:

  • To systematically review the development of DNA origami-based single-molecule detection.
  • To explore design principles for functional DNA nanostructures.
  • To analyze various readout strategies for achieving true single-molecule detection.

Main Methods:

  • Engineering DNA nanostructures like conformational switches and plasmonic hotspots.
  • Utilizing non-fluorescence-based approaches: AFM, nanopores, and optical nanoantennas with SERS.
  • Employing fluorescence-based approaches: dynamic DNA nanostructures and optical nanoantennas with fluorescent readout.

Main Results:

  • DNA origami enables precise spatial arrangement for functional nanomachines.
  • Various nanoarchitectures and readout strategies have been developed for single-molecule detection.
  • Key trends and technological gaps in the field are identified.

Conclusions:

  • DNA origami is a powerful tool for advancing single-molecule detection technologies.
  • Coupling engineered nanoarchitectures with tailored readout strategies is crucial.
  • Further research is needed to bridge technology gaps for next-generation applications.