Related Experiment Video
Updated: Jun 27, 2026

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
Published on: November 30, 2014
The Lipophorin Receptor Gene GdLpR Regulates Reproductive Diapause in Galeruca daurica
Ling Li1, Zhihan Yao1, Haichao Wang1
1Research Center for Grassland Entomology, Inner Mongolia Agricultural University, Hohhot 010018, China.
Abstract:
Lipophorin receptors play a crucial role in the reproductive development of insects. However, their regulatory function in insect reproductive diapause remains poorly understood. In this study, a lipophorin receptor gene (GdLpR) was cloned from Galeruca daurica using RT-PCR. The open reading frame (ORF) of GdLpR is 2589 bp in length, encoding 862 amino acids, and possesses typical structural characteristics of the lipophorin receptor family. RT-qPCR analysis indicated that the expression of GdLpR is up-regulated in G. daurica adults before diapause, exhibits an expression pattern of initial down-regulation, subsequent up-regulation, and then further down-regulation during diapause, and is up-regulated again after diapause termination. After RNA interference of GdLpR, the expression levels of diapause-related genes ecdysone receptor (EcR), nuclear hormone receptor (HR3), and vitellogenin (Vg) were downregulated, while the expression level of fatty acid synthase (FAS) and total lipid content were upregulated, leading to premature diapause in adult G. daurica. These results indicate that GdLpR is involved in the regulation of reproductive diapause in G. daurica.
Related Concept Videos
Background and Environment Affect Phenotype
An example of how genetic background affects phenotype can be seen in horses. The Extension gene in horses is responsible for their coat color. A wild-type gene (EE) produces black pigment in the coat, while a mutant gene (ee) produces red pigment. A...
The Ratio of X Chromosome to Autosomes
Normal male Drosophila has a ratio of one X chromosome to two sets of autosomes. In contrast, normal female Drosophila...
Dosage Compensation
In addition to sexual development, the X chromosome has genes involved in autosomal functions such as brain development and the immune system. Therefore, males and females with distinct numbers of X chromosomes will have...

