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Time-Resolved Whole-Transcriptome Analysis Suggests Candidate Non-Coding RNA Regulatory Networks Associated with
Hanbo Zhao1, Lei Liu2, Bin Yang3
1Kunpeng Institute of Modern Agriculture at Foshan, Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518124, China.
Insects
|June 26, 2026
Summary
This study reveals how non-coding RNAs regulate sex pheromone biosynthesis in moths after pheromone biosynthesis-activating neuropeptide (PBAN) treatment. A novel competing RNA network involving key genes was identified, offering insights into this crucial biological process.
Area of Science:
- Insect reproductive biology
- Molecular endocrinology
- Non-coding RNA regulation
Background:
- Sex pheromone biosynthesis in Lepidoptera is regulated by pheromone biosynthesis-activating neuropeptide (PBAN) signaling.
- The role of non-coding RNAs in post-transcriptional regulation of pheromone production is not well understood.
Purpose of the Study:
- To characterize temporal transcriptomic changes following PBAN treatment in *Ostrinia furnacalis*.
- To identify non-coding RNA-mediated regulatory associations and molecular dynamics.
- To elucidate the transcriptional remodeling process after PBAN stimulation.
Main Methods:
- Comprehensive whole-transcriptome sequencing (WTS) across six time points post-PBAN injection.
- Identification and quantification of differentially expressed mRNAs, miRNAs, lncRNAs, and circRNAs.
- Integrative analysis of multi-omics data to infer a competing endogenous RNA (ceRNA) network.
Main Results:
- Dynamic expression patterns of mRNAs, miRNAs, lncRNAs, and circRNAs were observed.
- A candidate ceRNA network was inferred, highlighting four core node genes: PBAN receptor (PBANR), acetyl-CoA carboxylase (ACC), desaturase (DES), and fatty acyl-CoA reductase (FAR).
- qRT-PCR validated the temporal expression patterns of key genes.
Conclusions:
- Non-coding RNAs play a significant role in the post-transcriptional regulation of sex pheromone biosynthesis.
- The identified ceRNA network provides a valuable resource for understanding PBAN-mediated regulation.
- Further functional studies can build upon these findings to explore gene regulation in pheromone production.
Keywords:
Ostrinia furnacalisPBAN-induced pheromone biosynthesiscompeting endogenous RNAnon-coding RNAwhole-transcriptome sequencingMore Related Videos
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