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Updated: Jun 27, 2026
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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
Published on: March 31, 2016
Transcriptomic Profiling of Adipose Tissues in Sujiang Pigs Reveals Candidate Genes Associated with Tissue-Specific
Huizhen Gao1, Shubin Zhu1, Ligang Ni1
1School of Modern Animal Husbandry, Jiangsu Agri-Animal Husbandry Vocational College, Taizhou 225300, China.
Life (Basel, Switzerland)
|June 26, 2026
Summary
Sujang pigs reveal distinct gene expression in subcutaneous and visceral fat. This study identifies key genes involved in lipid metabolism and inflammation, offering insights into adipose tissue regulation.
Area of Science:
- Animal Science
- Genomics
- Metabolic Research
Background:
- Adipose tissue is crucial for energy storage, metabolism, and inflammation.
- Sujang pigs, a Chinese breed, exhibit significant fat deposition, making them a valuable model for adipose tissue research.
Purpose of the Study:
- To investigate molecular differences between subcutaneous and visceral adipose tissues in Sujiang pigs.
- To identify candidate genes associated with lipid metabolism and inflammatory responses in porcine adipose tissue.
Main Methods:
- RNA-sequencing (RNA-seq) was performed on back fat (BF), greater omentum (GOM), and mesenteric adipose tissue (MAD) from Sujiang pigs.
- Differential gene expression analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted.
- Protein-protein interaction (PPI) networks were integrated with bioinformatics analyses to identify candidate genes.
Main Results:
- Comparative analyses revealed thousands of differentially expressed genes (DEGs) between BF and GOM (3005), BF and MAD (975), and GOM and MAD (892).
- RT-qPCR validated the reliability of the RNA-seq data for selected DEGs.
- Candidate genes linked to lipid metabolism (WNT9A, WNT5A, PDGFRA) and inflammation (CSF1R, C1QB, CD4) were identified.
Conclusions:
- Significant molecular distinctions exist between porcine visceral and subcutaneous adipose tissues.
- The identified candidate genes provide a foundation for understanding the molecular regulation of adipose tissue metabolism and inflammation in pigs.
