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Related Experiment Video

Updated: Jun 27, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
11:17

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

Published on: August 30, 2018

Simultaneous Quantification of Colistin A and B in Human Plasma Using a Small Volume with a High-Throughput LC-MS/MS

Jimin Yoon1, Won Gun Kwack2, Kyung-Tae Lee1,3,4

  • 1Department of Pharmacy, College of Pharmacy, Kyung Hee University, Seoul 02447, Republic of Korea.

Pharmaceuticals (Basel, Switzerland)
|June 26, 2026
PubMed
Summary

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A new bioanalytical assay precisely quantifies colistin A and B in plasma. This method supports therapeutic drug monitoring (TDM) for optimized colistin dosing and patient care.

Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Clinical Pharmacy

Background:

  • Colistin, a polymyxin antibiotic, has a narrow therapeutic window and high pharmacokinetic variability.
  • Existing analytical methods for colistin may lack precision due to using mixtures or requiring large sample volumes.
  • Accurate quantification of individual colistin components is crucial for effective therapeutic drug monitoring.

Purpose of the Study:

  • To develop and validate a sensitive, component-specific bioanalytical assay for simultaneous quantification of colistin A and colistin B in human plasma.
  • To enable precise measurement of individual colistin components for pharmacokinetic assessments.
  • To facilitate therapeutic drug monitoring (TDM)-guided dose optimization.

Main Methods:

  • Development of a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using component-specific standards.
Keywords:
LC–MS/MScolistincolistin Acolistin Bpharmacokineticstherapeutic drug monitoring

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Last Updated: Jun 27, 2026

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
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  • Solid-phase extraction from a small plasma volume (50 µL).
  • Rapid chromatographic separation (4 min) and detection via negative-ion multiple reaction monitoring (MRM).

    • Main Results:

    • Excellent linearity (R² > 0.99) and validated precision/accuracy for colistin A and B quantification (0.1-20 µg/mL).
    • Clinical validation in 60 plasma samples from 15 patients confirmed applicability in the relevant therapeutic range.
    • Demonstrated ability to capture individual concentration-time profiles.

    Conclusions:

    • A validated LC-MS/MS assay enables precise, high-throughput pharmacokinetic assessment of colistin A and B.
    • The assay utilizes a small plasma volume, making it practical for clinical use.
    • This tool supports TDM-guided colistin dose optimization and clinical research.