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Updated: Jun 27, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

Ultrasensitive Label-Free Electrochemical Detection of Pseudomonas aeruginosa Using a Surface Molecularly Imprinted

Naphatsawan Vongmanee1, Jindapa Nampeng1, Chuchart Pintavirooj1

  • 1Department of Biomedical Engineering, School of Engineering, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand.

Polymers
|June 26, 2026
PubMed

Insights

A new biomimetic sensor rapidly detects Pseudomonas aeruginosa, a common cause of hospital infections. This innovative method uses a surface-imprinted polymer for highly sensitive and selective bacterial identification, aiding early diagnosis and infection control.

Area of Science:

  • Biomaterials Science
  • Analytical Chemistry
  • Microbiology

Background:

  • * *Pseudomonas aeruginosa* is a significant opportunistic pathogen causing difficult-to-treat nosocomial infections.
  • * Antibiotic resistance and biofilm formation complicate *P. aeruginosa* infections.
  • * Rapid and selective detection is crucial for effective clinical management and infection control.

Purpose of the Study:

  • * To develop a novel electrochemical sensor for the rapid and selective detection of *Pseudomonas aeruginosa*.
  • * To utilize a surface-imprinted polymer (MIP) strategy with a multi-monomer system for enhanced bacterial recognition.

Main Methods:

  • * Fabrication of a surface-imprinted MIP using methacrylamide (MAM), acrylamide (AAM), and vinylpyrrolidone (VP) monomers.
  • * Development of an electrochemical sensor based on the MIP.
  • * Evaluation of sensor performance, including limit of detection (LOD), linear range, and reproducibility.

Main Results:

  • * Achieved an ultralow LOD of 1 CFU/mL for *P. aeruginosa* detection.
  • * Demonstrated a wide linear range of 1-104 CFU/mL with excellent analytical performance.
  • * Exhibited good sensor reproducibility with a relative standard deviation (RSD) of 5-12%.

Conclusions:

  • * The novel surface-imprinted MIP electrochemical sensor provides a rapid, cost-effective, and portable platform for *P. aeruginosa* detection.
  • * The multi-monomer system and surface imprinting strategy enhance bacterial recognition and sensing capabilities.
  • * The sensor shows strong potential for application in clinical diagnostics and environmental monitoring.
Keywords:
Pseudomonas aeruginosacyclic voltammetryelectrochemical biomimetic sensormolecularly imprinted polymernosocomial infectionscreen-printed electrodeultrasensitive detection

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