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Related Concept Videos

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
RNA Interference01:23

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

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Related Experiment Video

Updated: Jun 27, 2026

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
08:55

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points

Published on: May 29, 2020

RNAi Power Targets in Insect Pests: Beyond Functional Validation to Biopesticide Development Potential.

Momana Jamil1,2, Shakil Ahmad3, Valeria Palma-Onetto4

  • 1School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China.

Plants (Basel, Switzerland)
|June 26, 2026
PubMed
Summary

RNA interference (RNAi) offers an eco-friendly alternative to synthetic pesticides for sustainable pest management. This review highlights key RNAi target genes and delivery methods for effective insect control in agriculture.

Keywords:
RNA interference (RNAi)biopesticidescrop yieldsfood securityinsect pest controlsustainable agriculture

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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects
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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

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Last Updated: Jun 27, 2026

RNA Interference in Aquatic Beetles as a Powerful Tool for Manipulating Gene Expression at Specific Developmental Time Points
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Double-stranded RNA Oral Delivery Methods to Induce RNA Interference in Phloem and Plant-sap-feeding Hemipteran Insects

Published on: May 4, 2018

Area of Science:

  • Agricultural Science
  • Entomology
  • Biotechnology

Background:

  • Global agriculture faces challenges from climate change, biodiversity loss, and population growth.
  • Synthetic pesticides are losing efficacy due to pest resistance and raise environmental concerns.
  • Sustainable and eco-conscious food production necessitates novel pest management strategies.

Purpose of the Study:

  • To systematically identify promising RNA interference (RNAi) target gene families for insect pest control.
  • To evaluate the broad applicability and potential impact of RNAi by focusing on genes with demonstrated efficacy in multiple insect species.
  • To discuss the transition of RNAi technology from laboratory research to practical field applications in agriculture.

Main Methods:

  • Systematic review of scientific literature on RNAi target genes in insect pests.
  • Selection of genes based on demonstrated RNAi efficacy across at least three different insect species.
  • Analysis of RNAi delivery methods and research gaps for agricultural applications.

Main Results:

  • Identification of key developmental genes as promising RNAi targets for insect pest management.
  • Evidence of broad applicability of RNAi across diverse insect species.
  • Discussion on the challenges and opportunities for scaling RNAi technology in agricultural settings.

Conclusions:

  • RNAi presents a sustainable and environmentally friendly alternative to traditional chemical pesticides.
  • Further research is needed to enhance the efficiency and scalability of RNAi for real-world agricultural systems.
  • This review supports the development of RNAi-based biopesticides for effective and sustainable pest control.